CHD1 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

CHD1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human CHD1
  Lys1531-Thr1710
  Accession # O14646
• Specificity: Detects human and mouse CHD1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human CHD5 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CHD1 Antibody [Alexa Fluor® 700]

• ATP-dependent helicase CHD1
• CHD1
• CHD-1
• chromodomain helicase DNA binding protein 1
• chromodomain-helicase-DNA-binding protein 1
• DKFZp686E2337
• EC 3.6.1
• EC 3.6.4.12

Background

CHD1 (Chromohelicase/ATPase DNA‑binding protein 1) is a 200‑220 kDa member of the SNF2/RAD54 helicase family of proteins. It is an ATP‑dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state. In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency. Human CHD1 is 1710 amino acids (aa) in length. It contains two Ser‑rich regions (aa 1‑70 and 117‑137), two Chromo (chromatin‑organizer‑modifer) domains (aa 272‑364 and 389‑452), a SNF2 family N‑terminal domain (aa 484‑763) and a C‑terminal helicase domain (aa 792‑943). There are at least 30 Ser/Thr phosphorylation sites. Over aa 1531‑1710, human CHD1 shares 92% aa identity with mouse CHD1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.