CHD1 Antibody (677616) [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, Raji human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF Membrane was ...read more
CHD1 was detected in immersion fixed BG01V human embryonic stem cells using Human CHD1 Monoclonal Antibody (Catalog # MAB6195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CHD1 Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, KO
Clone
677616
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CHD1 Antibody (677616) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CHD1
Lys1531-Thr1710
Accession # O14646
Specificity
Detects human CHD1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CHD1L, 5, or 7 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CHD1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
Control
CHD1 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CHD1 Antibody (677616) [Unconjugated]

  • ATP-dependent helicase CHD1
  • CHD1
  • CHD-1
  • chromodomain helicase DNA binding protein 1
  • chromodomain-helicase-DNA-binding protein 1
  • DKFZp686E2337
  • EC 3.6.1
  • EC 3.6.4.12

Background

CHD1 (Chromohelicase/ATPase DNA-binding protein 1) is a 200-205 kDa member of the SNF2/RAD54 helicase family of proteins.  It is an ATP-dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state.  In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency.  Human CHD1 is 1710 amino acids (aa) in length.  It contains two Ser-rich regions (aa 1-70 and 117‑137), two Chromo (chromatin-organizer-modifer) domains (aa 272-364 and 389-452), a SNF2 family N-terminal domain (aa 484-763) and a C-terminal helicase domain (aa 792-943).  There are at least 30 Ser/Thr phosphorylation sites.  Over aa 1531-1710, human CHD1 shares 92% aa identity with mouse CHD1.    

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol CHD1
Uniprot