CHD1 Antibody (677616) [Alexa Fluor® 488]

• Applications: WB, ICC/IF, KO
• Clone: 677616
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

CHD1 Antibody (677616) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human CHD1
  Lys1531-Thr1710
  Accession # O14646
• Specificity: Detects human CHD1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CHD1L, 5, or 7 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CHD1 (Chromohelicase/ATPase DNA-binding protein 1) is a 200-205 kDa member of the SNF2/RAD54 helicase family of proteins.  It is an ATP-dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state.  In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency.  Human CHD1 is 1710 amino acids (aa) in length.  It contains two Ser-rich regions (aa 1-70 and 117‑137), two Chromo (chromatin-organizer-modifer) domains (aa 272-364 and 389-452), a SNF2 family N-terminal domain (aa 484-763) and a C-terminal helicase domain (aa 792-943).  There are at least 30 Ser/Thr phosphorylation sites.  Over aa 1531-1710, human CHD1 shares 92% aa identity with mouse CHD1.    

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.