CHAF1B Antibody (JG92-30)

Western Blot: CHAF1B Antibody (JG92-30) [NBP2-75439] - Analysis of p60 CAF1 on different cell lysates using anti-p60 CAF1 antibody at 1/1,000 dilution.Positive control:Lane 1: SiHa Lane 2: K562Lane 3: A431

Immunocytochemistry/Immunofluorescence: CHAF1B Antibody (JG92-30) [NBP2-75439] - Staining p60 CAF1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CHAF1B Antibody (JG92-30) [NBP2-75439] - Analysis of paraffin-embedded human appendix tissue using anti-p60 CAF1 antibody. Counter stained with hematoxylin.

Flow Cytometry: CHAF1B Antibody (JG92-30) [NBP2-75439] - Analysis of K562 cells with p60 CAF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CHAF1B Antibody (JG92-30) [NBP2-75439] - Staining p60 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CHAF1B Antibody (JG92-30) [NBP2-75439] - Staining p60 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CHAF1B Antibody (JG92-30) [NBP2-75439] - Analysis of paraffin-embedded human tonsil tissue using anti-p60 CAF1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CHAF1B Antibody (JG92-30) [NBP2-75439] - Analysis of paraffin-embedded human colon tissue using anti-p60 CAF1 antibody. Counter stained with hematoxylin.

Product Discontinued

CHAF1B Antibody (JG92-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human CHAF1B aa 419-559 / 559. (SwissProt: Q13112 Human)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CHAF1B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CHAF1B Antibody (JG92-30)

• CAF1
• CAF-1
• CAF1ACAF-1 subunit B
• CAF1P60CAF-I 60 kDa subunit
• CAF-IP60
• chromatin assembly factor 1 subunit B
• chromatin assembly factor 1, subunit B (p60)
• Chromatin assembly factor I p60 subunit
• human chromatin assembly factor-I p60 subunit, 10
• MPHOSPH7CAF-I p60
• MPP7M-phase phosphoprotein 7

Background

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

CAF-1 antibodies are useful tools for cell division, DNA replication and DNA packaging.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHAF1B