CGI-09 Antibody - Azide and BSA Free

Western Blot: CGI-09 Antibody [NBP2-92578] - Analysis of extracts of various cell lines, using CGI-09 at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CGI-09 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CGI-09 Antibody - Azide and BSA Free (NBP2-92578) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human TRMT6 (NP_057023.2). MEGSGEQPGPQPQHPGDHRIRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKKREEPTAETKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEIVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHMRYDTLAQMLTLGNIRAG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRMT6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for CGI-09 Antibody - Azide and BSA Free

• CGI-09
• GCD10
• Gcd10p
• KIAA1153
• MGC5029
• TRM6
• tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6
• tRNA methyltransferase 6 homolog (S. cerevisiae)
• tRNA(m1A58)-methyltransferase subunit TRM6
• tRNA(m1A58)MTase subunit TRM6

Background

CGI-09 is encoded by this gene is similar to an uncharacterized protein from C. elegans. Although the function of the encoded protein is unknown, it shares weak sequence similarity to a region of S. cerevisiae translation initiation factor subunit Gcd10p.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: TRMT6