cGAS Antibody

Images

 
Genetic Strategies: Western Blot: cGAS Antibody [NBP1-86761] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-MB21D1 antibody. Remaining relative intensity is ...read more
Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in lymphoid cells.
Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in Hoffbauer cells and placental trophoblast.
Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Immunohistochemical staining of human duodenum shows moderate to strong cytoplasmic positivity in lymphoid cells in lamina propria.
Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Immunohistochemical staining of human lung shows moderate to strong cytoplasmic positivity in macrophages and a subset of leukocytes.
Simple Western: cGAS Antibody [NBP1-86761] - Simple Western lane view shows a specific band for MB21D1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Simple Western: cGAS Antibody [NBP1-86761] - Electropherogram image(s) of corresponding Simple Western lane view. MB21D1 antibody was used at 1:30 dilution on RT-4 lysate(s).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

cGAS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RKQLRLKPFYLVPKHAKEGNGFQEETWRLSFSHIEKEILNNHGKSKTCCENKEEKCCRKDCLKLMKYLLEQLKERF
Specificity
Specificity of human cGAS antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MB21D1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Simple Western 1:30
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ICC/IF reactivity reported in scientific literature (PMID: 24970844]).
Control Peptide
cGAS Recombinant Protein Antigen (NBP1-86761PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-86761 in the following applications:

Publications
Read Publications using
NBP1-86761 in the following applications:

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24284630)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for cGAS Antibody

  • C6orf150
  • c-GAS
  • cyclic GMP-AMP synthase
  • h-cGAS
  • Mab-21 domain containing 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for cGAS Antibody (NBP1-86761)(3)

Review for cGAS Antibody (NBP1-86761) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-86761:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot cGAS NBP1-86761
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reviewed by:
Anonymous
WB Human 12/18/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman primary hepatocytes
SpeciesHuman
LotG105753

Comments

CommentsName: Anti-cGAS antibody (NBP1-86761)
Catalog #: Anti-cGAS antibody (NBP1-86761)
Lot Number: Anti-cGAS antibody (NBP1-86761, Lot # G105753)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): lane 1: human primary hepatocytes; lane 2: Hep G2
Sample Information:
     Cell Line or Tissue: human primary hepatocytes, Hep G2
Species: human
     Treatment: No
Lysate Preparation:
     Date of lysate preparation: December 7, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: No
     Negative Control: No
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 10%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/500
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times
Secondary Antibody
Manufacturer and Catalog #: Promega, cat # W401B, Lot # 0000187662
Secondary description: goat anti-rabbit secondary antibody
     Dilution: 1/2000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot #n9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 3 min
Molecular weight of band(s): ~55 kDa
Experimental Concerns and Observations: Specific bands around 55 kDa were observed

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for cGAS Antibody (NBP1-86761) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for cGAS Antibody (NBP1-86761)

Discover related pathways, diseases and genes to cGAS Antibody (NBP1-86761). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for cGAS Antibody (NBP1-86761)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
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Anonymous
12/18/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol MB21D1