Genetic Strategies: Western Blot: cGAS Antibody [NBP1-86761] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-MB21D1 antibody. Remaining relative intensity is ...read more
Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells
Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human tonsil shows moderate to strong granular cytoplasmic positivity in non-germinal center and germinal center cells.
Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.
Simple Western: cGAS Antibody [NBP1-86761] - Simple Western lane view shows a specific band for MB21D1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Simple Western: cGAS Antibody [NBP1-86761] - Electropherogram image(s) of corresponding Simple Western lane view. MB21D1 antibody was used at 1:30 dilution on RT-4 lysate(s).
Name: Anti-cGAS antibody (NBP1-86761) Catalog #: Anti-cGAS antibody (NBP1-86761) Lot Number: Anti-cGAS antibody (NBP1-86761, Lot # G105753) PO/Order Number: Click here to enter text.. WB Image Description (Please provide labels for all lanes): lane 1: human primary hepatocytes; lane 2: Hep G2 Sample Information: Cell Line or Tissue: human primary hepatocytes, Hep G2 Species: human Treatment: No Lysate Preparation: Date of lysate preparation: December 7, 2017 Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF Reducing agent: beta-mercaptoethanol, DTT If boiled (temperature/time): Yes Controls: Positive Control: No Negative Control: No Loading Control (please attach additional images if applicable): No Protein Amount Loaded per lane: 20 ug Antibody Storage Conditions: -20℃ Electrophoresis: Gel Percentage: 10% Electrophoresis Conditions: Tris-Glycine-SDS at room temperature Voltage: 120V Time: 2 hours Membrane Transfer: Method (Submersion/Semi-dry): wet transfer Membrane Type (PVDF/Nitrocellulose): Nitrocellulose Time: 2 hours Voltage: 100V Blocking: Blocking Solution: 5% milk in 1X TBST Time: 1 hour at room temperature Primary Antibody: Dilution: 1/500 Diluent Buffer: 2.5% BSA Incubation Time: overnight Incubation Temperature: 4℃ Washing Conditions: Wash Solution: 1X TBST Time and Repetitions: 5 min each for 3 times Secondary Antibody Manufacturer and Catalog #: Promega, cat # W401B, Lot # 0000187662 Secondary description: goat anti-rabbit secondary antibody Dilution: 1/2000 Diluent Buffer: 3% milk Incubation Time: 1 hour Incubation Temperature: room temperature Detection Method: Detection: ECL (GE, cat # RPN2209, lot #n9838243) Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure Development Time: 3 min Molecular weight of band(s): ~55 kDa Experimental Concerns and Observations: Specific bands around 55 kDa were observed
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