Home » CEP164 » CEP164 Antibodies » CEP164 Antibody (17)

CEP164 Antibody (17)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: CEP164 Antibody (17) [NBP2-43707] - Analysis of whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Cep 164 antibody diluted by 1:10000.
Western Blot: CEP164 Antibody (17) [NBP2-43707] - Analysis of whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Cep 164 antibody diluted by 1:10000.
Western Blot: CEP164 Antibody (17) [NBP2-43707] - Analysis of 30 ug whole cell lysate. A: HeLa 5% SDS PAGE diluted at 1:1000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
17
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

CEP164 Antibody (17) Summary

Immunogen
The exact sequence is proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CEP164
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:20000
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
164 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for CEP164 Antibody (17)

  • centrosomal protein 164kDa
  • FLJ54767

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

ATM Antibody (2C1)
NB100-309
Western Blot: ATM Antibody (2C1) [NB100-309] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:1000. Theoretical molecular weight 351 kDa.
Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody (2C1) [NB100-309] at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western Blot: ATM Antibody (2C1) [NB100-309] - Detection of human ATM protein using ATM antibody (2C1) [NB100-309] by western blot or immunoprecipitation. Theoretical molecular weight 351 kDa.
(3 Reviews)
(120 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD
Chk1 Antibody
NB100-464
Western Blot: Chk1 Antibody [NB100-464] - Knockout of ORC2 in HCT116 p53-/- cells. Western blot of ORC2 in HBEC and 293T cell lines. Ponceau S staining of total protein or immunoblot of Chk1 show equal loading of the pairs of lanes. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/19084), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Chk1 Antibody [NB100-464] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Chk1 Antibody [NB100-464] - Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody NB100-464 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(8 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
ATR Antibody (2B5)
NB100-308
Western Blot: ATR Antibody (2B5) [NB100-308] - Human Raji whole cell extract. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: ATR Antibody (2B5) [NB100-308] - Analysis of HeLa, using antibody at 1:100 dilution.
Western Blot: ATR Antibody (2B5) [NB100-308] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] diluted at 1:500.
(18 Publications)
Species: Hu, Mu
Applications: WB, Func, ICC/IF, IP, In vitro, KO
TEM8/ANTXR1
Antibody (200C1339(SB
NB100-56585
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection in TEM8 transfected cell lysate using this antibody.
Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Detection of TEM8/ANTXR1 in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.
Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) [NB100-56585] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody (NB100-56585) at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).
(5 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MMAB Antibody
(OTI2G5)
NBP2-03417
Western Blot: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB.
Immunohistochemistry-Paraffin: MMAB Antibody (2G5) [NBP2-03417] Staining of paraffin-embedded Human lymph node tissue using anti-MMAB mouse monoclonal antibody.
Flow Cytometry: MMAB Antibody (2G5) [NBP2-03417] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody, and then analyzed by flow cytometry.
Species: Hu
Applications: WB, Flow, IHC, IHC-P
POLR1A Antibody
NBP2-32016
Immunohistochemistry-Paraffin: POLR1A Antibody [NBP2-32016] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: POLR1A Antibody [NBP2-32016] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: POLR1A Antibody [NBP2-32016] - Staining of human kidney shows strong nuclear positivity in cells in tubules.
Species: Hu
Applications: IHC, IHC-P
CNTLN Antibody
NBP1-88795
Western Blot: CNTLN Antibody [NBP1-88795] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: CNTLN Antibody [NBP1-88795] - Staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.
Species: Hu
Applications: WB, IHC, IHC-P
ZNF423 Antibody
NBP2-76351
Immunocytochemistry/Immunofluorescence: ZNF423 Antibody [NBP2-76351] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: ZNF423 Antibody [NBP2-76351] - Analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Species: Hu
Applications: Flow, ICC/IF, PEP-ELISA
ARL13B Antibody
NBP2-15463
Western Blot: ARL13B Antibody [NBP2-15463] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: ARL13B Antibody [NBP2-15463] - The presence of VDAC1-DC in ccRCC cells decreases or abolishes ciliation. Triple immunofluorescence labeling and merged images with acetylated alpha -tubulin (Acet. alpha -tubulin in red), Arl13b (in green) and DAPI (in blue). Image collected and cropped by CiteAb from the following publication (//www.thno.org/v10p2696.htm) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: ARL13B Antibody [NBP2-15463] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Chk2 Antibody
(73C175.1.1)
NB100-56546
Western Blot: Chk2 Antibody (73C175.1.1) [NB100-56546] - Western Blot: Chk2 Antibody (73C175.1.1) - Azide Free [NBP2-27403] - analysis of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with Chk2 antibody at 2 ug/ml. Image using the Azide Free form of this antibody.
Immunocytochemistry/Immunofluorescence: Chk2 Antibody (73C175.1.1) [NB100-56546] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Chk2 (73C175.1.1) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Chk2 Antibody (73C175.1.1) [NB100-56546] - Staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
MDC1 Antibody
NB100-395
Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NB100-395] - PARP activity affects 53BP1 recruitment to low-input power damage. Immunofluorescent localization of MDC1 and 53BP1 in HeLa cells irradiated with 15% (indicated by white arrowheads) and/or 30% (indicated by yellow arrowheads) at 30 min p.i. N = 10 with consistent results. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv976), licensed under a CC-BY licence.
Western Blot: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
(2 Reviews)
(17 Publications)
Species: Hu, Mu
Applications: WB, ICC/IF, IP
RPA70 Antibody
NB100-2204
Western Blot: RPA70 Antibody [NB100-2204] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiRPA70 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - Human ovarian carcinoma. Antibodies: Affinity purified rabbit anti- RPA70 Cat. No.used at a dilution of 1:1,000 (1 ug/ml) (left) and used at a dilution of 1:5,000 (0.2ug/ml (right). Detection: DAB
Western Blot: RPA70 Antibody [NB100-2204] - Detection of Human RPA70 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa and (15 and 50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA70 antibody NB100-2204 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(6 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
Nbs1 Antibody
NB100-143
Knockout Validated: Nbs1 Antibody [NB100-143] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and Nbs1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Polyclonal [NB100-143] followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for Nbs1 at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Nbs1 Antibody [NB100-143] - Western Blot with Nbs1 Antibody [NB100-143]. KSHV LANA recruits MRN (Mre11-Rad50-NBS1) complex. Co-immunoprecipitation of endogenous LANA and MRN proteins in BC3 cells. Cells were lysed using TBS-T buffer and the cell lysate was incubated with benzonase. After centrifugation, supernatant was incubated overnight with anti-LANA or IgG-control beads. The precipitated complexes were analyzed for the presence of endogenous Rad50, Mre11 and NBS1 by SDS-PAGE and immunoblotting. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.ppat.1006335), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - ICC/IF analysis of Nbs1 in HeLa cells with Nbs1 Antibody [NB100-143]. Image from verified customer review.
(8 Reviews)
(178 Publications)
Species: Hu, Mu, Ha, Ma, Pm
Applications: WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, ISH, PAGE, KO
NLRP2/NALP2
Antibody
NB100-56155
Western Blot: NLRP2/NALP2 Antibody [NB100-56155] - Analysis of NLRP2/NALP2 using antibody at 1:2000. HEK293T: mock transfected cells, HEK293T-NALP2:NALP2 transiently transfected cells; THP-1: untreated cells; THP1-LPS: cells treated with 1 ug/ml LPS for4h. MCF-7: untreated cells.
Immunohistochemistry-Paraffin: NLRP2/NALP2 Antibody [NB100-56155] - NLRP2 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human NLRP2 polyclonal Antibody (Catalog # NB100-56155) at 1:500 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in horionic villi.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P, IP
Cyclin D2 Antibody
H00000894-B01P
Western Blot: Cyclin D2 Antibody [H00000894-B01P] - Analysis of CCND2 expression in human placenta.
Western Blot: Cyclin D2 Antibody [H00000894-B01P] - Analysis of CCND2 expression in A-431.
Western Blot: Cyclin D2 Antibody [H00000894-B01P] - Analysis of CCND2 expression in transfected 293T cell line by CCND2 polyclonal antibody. Lane 1: CCND2 transfected lysate(31.79 KDa). Lane 2: Non-transfected lysate.
(1 Publication)
Species: Hu, Rt
Applications: WB
Histone H2AX [p
Ser139] Antibody [Unc ...
AF2288
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM camptothecin (CPT) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Histone H2AX (S139) at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 24 hours, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Histone H2AX (S139) at approximately 25 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Histone H2AX phosphorylated at S139 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with ultraviolet radiation using Rabbit Anti-Human Phospho-Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2288) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(12 Publications)
Species: Hu
Applications: WB, Simple Western, IHC, ICC
Calnexin Antibody
NB100-1965
Western Blot: Calnexin Antibody [NB100-1965] - Subcellular localization of TM6SF2.Immunoaffinity isolation of ER and Golgi complex from mouse liver. ER and Golgi fractions were prepared from mouse liver microsomes by immunoaffinity chromatography as described under Experimental Procedures. Microsome membranes were dissolved in RIPA buffer, and equal volumes were separated on 10% SDS-PAGE and immunoblotting as described under Experimental Procedures. BiP, binding immunoglobulin protein; Gos28, Golgi SNAP receptor complex member 1; *, nonspecific band. Image collected and cropped by Cite Ab from the following publication (Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins. J Biol Chem (2016) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NB100-1965] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.
(1 Review)
(24 Publications)
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, Gp, Rb, Sh, Xp, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Ninein Antibody
NBP2-93990
Western Blot: Ninein Antibody [NBP2-93990] - Analysis of extracts of Jurkat cells, using Ninein.Exposure time: 90s.
Immunocytochemistry/Immunofluorescence: Ninein Antibody [NBP2-93990] - Analysis of U2OS cells using Ninein at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Ninein Antibody [NBP2-93990] - Paraffin-embedded rat kidney using Ninein.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for CEP164 Antibody (NBP2-43707) (0)

There are no publications for CEP164 Antibody (NBP2-43707).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CEP164 Antibody (NBP2-43707) (0)

There are no reviews for CEP164 Antibody (NBP2-43707). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CEP164 Antibody (NBP2-43707) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional CEP164 Products

Bioinformatics Tool for CEP164 Antibody (NBP2-43707)

Discover related pathways, diseases and genes to CEP164 Antibody (NBP2-43707). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CEP164 Antibody (NBP2-43707)

Discover more about diseases related to CEP164 Antibody (NBP2-43707).
 

Pathways for CEP164 Antibody (NBP2-43707)

View related products by pathway.

PTMs for CEP164 Antibody (NBP2-43707)

Learn more about PTMs related to CEP164 Antibody (NBP2-43707).

Blogs on CEP164

There are no specific blogs for CEP164, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  PDF Datasheet
  Request CofA
  Request SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CEP164 Antibody (17) and receive a gift card or discount.


Bioinformatics

Gene Symbol CEP164