Centaurin alpha 1 Antibody

Western Blot: Centaurin alpha 1 Antibody [NB300-907] - Staining of Human Cerebellum (A), Mouse (B), Rat (C) and Pig (D) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Centaurin alpha 1 Antibody Summary

• Immunogen: Peptide with sequence C-QEYAVEAHFKHKP corresponding to C-Terminus according to NP_006860.1.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ADAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human brain and mouse brain lysates (calculated MW of 43.4 kDa band according to NP_006860).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Centaurin alpha 1 Antibody

• arf-GAP with dual PH domain-containing protein 1
• ArfGAP with dual PH domains 1
• CENTA1centaurin-alpha-1
• centaurin, alpha 1
• centaurin-alpha
• Centaurin-alpha-1
• cnt-a1
• GCS1L
• p42IP4
• Putative MAPK-activating protein PM25

Background

The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Centaurin alpha 1 Antibody (NB300-907)(1)

Publication using NB300-907

• Venkateswarlu K, Oatey PB, Tavare JM et al. Identification of centaurin-alpha1 as a potential in vivo phosphatidylinositol 3,4,5-trisphosphate-binding protein that is functionally homologous to the yeast ADP-ribosylation factor (ARF) GTPase-activating protein, Gcs1. Biochem J 1999-06-01 [PMID: 10333475]

Bioinformatics

• Gene Symbol: ADAP1
• Entrez:
  • 11033(Human)
  • 231821(Mouse)
  • 171097(Rat)
• Uniprot:
  • NP_006860.(Human)