CENPH Antibody (1F7)

Images

 
Western Blot: CENPH Antibody (1F7) [H00064946-M01] - Analysis of CENPH expression in transfected 293T cell line by CENPH monoclonal antibody (M01), clone 1F7.Lane 1: CENPH transfected lysate(28.5 KDa).Lane 2: ...read more
Sandwich ELISA: CENPH Antibody (1F7) [H00064946-M01] - Detection limit for recombinant GST tagged CENPH is 0.03 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CENPH Antibody (1F7) Summary

Immunogen
CENPH (AAH12024, 1 a.a. ~ 248 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
CENPH - centromere protein H (1F7)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CENPH
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CENPH Antibody (1F7)

  • CENP-H
  • centromere protein H
  • ICEN35
  • Interphase centromere complex protein 35
  • kinetochore protein CENP-H
  • NNF1
  • PMF1

Background

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CENPH
Entrez
Uniprot