Rat CEBP Delta ELISA Kit (Colorimetric)

ELISA: Rat CEBP Delta ELISA Kit (Colorimetric) [NBP2-70027] - Samples were spiked with high concentrations of Rat CEBP Delta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat CEBP Delta ELISA Kit (Colorimetric) [NBP2-70027] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Rat CEBP Delta ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat C/EBP(delta) in samples. No significant cross-reactivity or interference between Rat C/EBPIdelta) and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.92%
• Intra-Assay: CV% < 5.58%
• Spike Recovery: 90-105%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CEBPD

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat CEBP Delta ELISA Kit (Colorimetric)

• C/EBP-delta
• CCAAT/enhancer binding protein (C/EBP), delta
• CCAAT/enhancer-binding protein delta
• CELF
• CRP3
• NF-IL6-betaC/EBP delta
• Nuclear factor NF-IL6-beta

Background

C/EBP proteins are DNA-binding proteins that are important transcriptional activators in the regulation of genes involved in immune and inflammatory responses and may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages. Members of the C/EBP transcription factor family are related by a high degree of amino acid sequence identity to the basic leucine zipper DNA-binding domain and show distinct but overlapping patterns of tissue- and stage-restricted expression. Osada et al. identified the consensus binding site for the family as RTTGCGYAAY (R = A or G, and Y = C or T). Phosphorylation of C/EBP delta may increase binding activity, whereas phosphorylation of the alpha and beta family members may decrease binding affinity. C/EBP delta is a nuclear protein that binds DNA as a dimer and can form stable heterodimers with all other C/EBP family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CEBPD