Human CEBP Beta ELISA Kit (Colorimetric)

ELISA: Human CEBP Beta ELISA Kit (Colorimetric) [NBP2-70025] - Samples were spiked with high concentrations of Human CEBP Beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human CEBP Beta ELISA Kit (Colorimetric) [NBP2-70025] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL

Human CEBP Beta ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human C/EBP(beta) in samples. No significant cross-reactivity or interference between Human C/EBP(beta) and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.22%
• Intra-Assay: CV% < 5.21%
• Spike Recovery: 86-110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CEBPB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CEBP Beta ELISA Kit (Colorimetric)

• C/EBP beta
• C/EBP-beta
• CCAAT/enhancer binding protein (C/EBP), beta
• CCAAT/enhancer-binding protein beta
• CRP2
• IL6DBP
• interleukin 6-dependent DNA-binding protein
• LAPTCF-5
• Liver activator protein
• liver-enriched transcriptional activator protein
• MGC32080
• NF-IL6
• Nuclear factor NF-IL6
• nuclear factor of interleukin 6
• TCF5NFIL6
• Transcription factor 5

Background

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth and differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP beta occurs predominantly as a heterodimer (3). C/EBP beta gamma readily heterodimerize with each other as well as with C/EBP alpha (4). Additionally, 3 isoforms of C/EBP beta can be detected (LAP, LAP,LIP)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CEBP Beta ELISA Kit (NBP2-70025). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CEBPB