![Western Blot: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12](http://images.novusbio.com/fullsize2/CEBP-Beta-Antibody-(SC55-05)-Western-Blot-NBP2-67635-img0004.jpg "Western Blot: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IP |
| Clone | SC55-05 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | C terminal Human C/EBP Beta |
| Localization | Cytoplasm, Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CEBPB |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Diseases for CEBP Beta Antibody (NBP2-67635)Discover more about diseases related to CEBP Beta Antibody (NBP2-67635).
| Pathways for CEBP Beta Antibody (NBP2-67635)View related products by pathway.
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PTMs for CEBP Beta Antibody (NBP2-67635)Learn more about PTMs related to CEBP Beta Antibody (NBP2-67635).
| Research Areas for CEBP Beta Antibody (NBP2-67635)Find related products by research area.
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Transcription Factor Antibodies Used In Landmark Evolutionary Study We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising... Read full blog post. |
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| Gene Symbol | CEBPB |
![Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/CEBP Beta Antibody (SC55-05)-Immunocytochemistry-Immunofluorescence-NBP2-67635-img0005.jpg "Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Flow Cytometry: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/CEBP-Beta-Antibody-(SC55-05)-Flow-Cytometry-NBP2-67635-img0001.jpg "Flow Cytometry: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/CEBP-Beta-Antibody-(SC55-05)-Immunocytochemistry-Immunofluorescence-NBP2-67635-img0002.jpg "Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/CEBP-Beta-Antibody-(SC55-05)-Immunocytochemistry-Immunofluorescence-NBP2-67635-img0003.jpg "Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Western Blot: LAP3 Antibody [NBP1-82847] - Analysis using Anti-LAP3 antibody NBP1-82847 (A) shows similar pattern to independent antibody NBP1-82848 (B).](http://images.novusbio.com/fullsize2/LAP3-Antibody-Western-Blot-NBP1-82847-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: LAP3 Antibody [NBP1-82847] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & midbody.](http://images.novusbio.com/fullsize2/LAP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82847-img0006.jpg)
![Immunohistochemistry-Paraffin: LAP3 Antibody [NBP1-82847] - Staining of human kidney.](http://images.novusbio.com/fullsize2/LAP3 Antibody-Immunohistochemistry-Paraffin-NBP1-82847-img0014.jpg)
![Western Blot: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of Phospho-c-Jun(S63) on different lysates using anti-Phospho-c-Jun(S63) antibody at 1/1,000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T](http://images.novusbio.com/fullsize2/c-jun-[p-Ser63]-Antibody-(SY0297)-Western-Blot-NBP2-67471-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Staining Phospho-c-Jun(S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/c-jun [p Ser63] Antibody (SY0297)-Immunocytochemistry-Immunofluorescence-NBP2-67471-img0006.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser63] Antibody (SY0297) [NBP2-67471] - Analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun(S63) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/c-jun-[p-Ser63]-Antibody-(SY0297)-Immunohistochemistry-Paraffin-NBP2-67471-img0004.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0016.jpg)
![Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Immunohistochemistry-NBP2-45731-img0013.jpg)
![Western Blot: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1.](http://images.novusbio.com/fullsize2/beta-Galactosidase-1-GLB1-Antibody-(1C9)-Western-Blot-NBP2-45731-img0014.jpg)
![Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize2/NFkB-p105-p50-Antibody-(2J10D7)-Western-Blot-NB100-56583-img0004.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review](http://images.novusbio.com/fullsize2/NFkB-p105-p50-Antibody-(2J10D7)-Immunohistochemistry-Paraffin-NB100-56583-img0009.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize2/NFkB-p105-p50-Antibody-(2J10D7)-Flow-Cytometry-NB100-56583-img0008.jpg)
![Western Blot: EBP Antibody [NBP1-59782] - Jurkat cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize2/EBP-Antibody-Western-Blot-NBP1-59782-img0002.jpg)
![Western Blot: SH3D19 Antibody (5C7) [H00152503-M01] - SH3D19 monoclonal antibody (M01), clone 5C7 Analysis of SH3D19 expression in HeLa.](http://images.novusbio.com/fullsize2/SH3D19-Antibody-(5C7)-Western-Blot-H00152503-M01-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SH3D19 Antibody (5C7) [H00152503-M01] - Analysis of monoclonal antibody to SH3D19 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/SH3D19-Antibody-(5C7)-Immunocytochemistry-Immunofluorescence-H00152503-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: SH3D19 Antibody (5C7) [H00152503-M01] - Analysis of monoclonal antibody to SH3D19 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/SH3D19-Antibody-(5C7)-Immunohistochemistry-Paraffin-H00152503-M01-img0008.jpg)
![Western Blot: c-Fos Antibody [NB110-75039] - Analysis on marmoset brain lysate prepared in Ripa buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000incubated at 4C overnight.](http://images.novusbio.com/fullsize2/c-Fos-Antibody-Western-Blot-NB110-75039-img0074.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NB110-75039] - Rat trigeminal using Rabbit antibody to cFos: whole serum at a 1:500 dilution.](http://images.novusbio.com/fullsize2/c-Fos-Antibody-Immunocytochemistry-Immunofluorescence-NB110-75039-img0063.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC on rat brain (paraffin section) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/fullsize2/c-Fos-Antibody-Immunohistochemistry-Paraffin-NB110-75039-img0072.jpg)
![Western Blot: ASRGL1 Antibody [NBP1-89133] - Analysis in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/ASRGL1-Antibody-Western-Blot-NBP1-89133-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: ASRGL1 Antibody [NBP1-89133] - Staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/ASRGL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89133-img0004.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP1-89133] - Staining of human cervix, uterine, endometrium, fallopian tube and skeletal muscle using Anti-ASRGL1 antibody NBP1-89133 (A) shows similar protein distribution across tissues to independent antibody NBP2-38759 (B).](http://images.novusbio.com/fullsize2/ASRGL1-Antibody-Immunohistochemistry-Paraffin-NBP1-89133-img0012.jpg)
![Western Blot: SMG1 Antibody (1A8) [H00023049-M02] - SMG1 monoclonal antibody (M02), clone 1A8 Analysis of SMG1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize2/SMG1 Antibody (1A8)-Western Blot-H00023049-M02-img0007.jpg)
![Immunohistochemistry-Paraffin: SMG1 Antibody (1A8) [H00023049-M02] - Analysis of monoclonal antibody to SMG1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/SMG1-Antibody-(1A8)-Immunohistochemistry-Paraffin-H00023049-M02-img0005.jpg)
![Sandwich ELISA: SMG1 Antibody (1A8) [H00023049-M02] - Detection limit for recombinant GST tagged SMG1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/SMG1-Antibody-(1A8)-Sandwich-ELISA-H00023049-M02-img0006.jpg)
![Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: Human C/EBPbeta. Lane 2: Mouse C/EBPdelta. Lane 3: untransfected. Lane 4: Human C/EBPdelta. 35 ug per lane. Primary: Anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize2/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0006.jpg)
![Western Blot: CEBP Delta Antibody [NB110-85519] - Detection of endogenous protein in whole cell extracts from mouse macrophage cells. Untreated WT cells (Lane 1), WT cells treated 3 hrs with LPS (Lane 2) and knock-out cells treated 3 hrs with LPS (Lane 3). The band at ~35 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of endogenous C/EBP delta. Primary antibody at 1:15,000. ECL was used for detection.](http://images.novusbio.com/fullsize2/CEBP-Delta-Antibody-Western-Blot-NB110-85519-img0001.jpg)
![Western Blot: CEBP Delta Antibody [NB110-85519] - Lane 1: untreated wild-type (WT) cells. Lane 2: WT cells treated 3 hrs with LPS. Lane 3: C/EBPd-knock-out cells treated 3 hrs with LPS. 35 ug per lane. Primary: anti-C/EBPd antibody at 1:15k overnight at 4C. Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, 35 kDa.](http://images.novusbio.com/fullsize2/CEBP Delta Antibody-Western Blot-NB110-85519-img0007.jpg)
![Western Blot: CEBP alpha Antibody (15C8) [NB600-1438] - Analysis of transfected 293T cells.](http://images.novusbio.com/fullsize2/CEBP alpha Antibody (15C8)-Western Blot-NB600-1438-img0003.jpg)
![Western Blot: JunB/AP-1 Antibody [NBP1-89544] - Analysis in human cell lines MCF-7 and HEK293 using anti-JUNB antibody. Corresponding JUNB RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.](http://images.novusbio.com/fullsize2/JunB-AP-1 Antibody-Western Blot-NBP1-89544-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody [NBP1-89544] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/JunB-AP-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89544-img0011.jpg)
![Immunohistochemistry-Paraffin: JunB/AP-1 Antibody [NBP1-89544] - Staining in human fallopian tube and skeletal muscle tissues. Corresponding JUNB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/JunB-AP-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89544-img0016.jpg)
![Western Blot: FosB/G0S3 Antibody (83B1138) [NB100-56530] - Analysis of FosB in HeLa cell lysate where bands are observed at ~38 kDa representing FosB and at ~35 kDa representing delta FosB.](http://images.novusbio.com/fullsize2/FosB-G0S3-Antibody-(83B1138)-Western-Blot-NB100-56530-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/JunD-[p-Ser255]-Antibody-(JJ08-21)-Immunocytochemistry-Immunofluorescence-NBP2-67861-img0003.jpg)
![Immunohistochemistry-Paraffin: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Analysis of paraffin-embedded mouse liver tissue using anti-Phospho-JunD(S255) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/JunD-[p-Ser255]-Antibody-(JJ08-21)-Immunohistochemistry-Paraffin-NBP2-67861-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/JunD-[p-Ser255]-Antibody-(JJ08-21)-Immunocytochemistry-Immunofluorescence-NBP2-67861-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")