CEBP Beta Antibody (SC55-05)

Western Blot: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Western blot analysis of CEBP Beta on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Ant

Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12

Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

CEBP Beta Antibody (SC55-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Rat CEBP Beta aa 248-297 / 297. (SwissProt: P17676 Human; SwissProt: P28033 Mouse; SwissProt: P21272 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CEBPB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CEBP Beta Antibody (SC55-05)

• C/EBP beta
• C/EBP-beta
• CCAAT/enhancer binding protein (C/EBP), beta
• CCAAT/enhancer-binding protein beta
• CRP2
• IL6DBP
• interleukin 6-dependent DNA-binding protein
• LAPTCF-5
• Liver activator protein
• liver-enriched transcriptional activator protein
• MGC32080
• NF-IL6
• Nuclear factor NF-IL6
• nuclear factor of interleukin 6
• TCF5NFIL6
• Transcription factor 5

Background

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth and differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). A number of serine targets of PKA have been identified in C/EBP, suggesting its activities are post-translationally regulated by cAMP (2). Even though, it has been show that C/EBP beta occurs predominantly as a heterodimer (3). C/EBP beta gamma readily heterodimerize with each other as well as with C/EBP alpha (4). Additionally, 3 isoforms of C/EBP beta can be detected (LAP, LAP,LIP)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEBPB