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CEBP Beta Antibody (SC55-05)

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Western Blot: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Western blot analysis of CEBP Beta on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Ant
Western Blot: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Western blot analysis of CEBP Beta on PC-12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room ...read more
Immunocytochemistry/ Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Flow Cytometry: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Analysis of Hela cells with C/EBP Beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; ...read more
Western Blot: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Analysis of C/EBP Beta on different lysates using anti-C/EBP Beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12
Immunocytochemistry/ Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: CEBP Beta Antibody (SC55-05) [NBP2-67635] - Staining C/EBP Beta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IP
Clone
SC55-05
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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CEBP Beta Antibody (SC55-05) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within C-terminal rat CEBP Beta. (SwissProt: P17676 Human;SwissProt: P28033 Mouse;SwissProt: P21272 Rat)
Localization
Cytoplasm, Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CEBPB
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CEBP Beta Antibody (SC55-05)

  • C/EBP beta
  • C/EBP-beta
  • CCAAT/enhancer binding protein (C/EBP), beta
  • CCAAT/enhancer-binding protein beta
  • CRP2
  • IL6DBP
  • interleukin 6-dependent DNA-binding protein
  • LAPTCF-5
  • Liver activator protein
  • liver-enriched transcriptional activator protein
  • MGC32080
  • NF-IL6
  • Nuclear factor NF-IL6
  • nuclear factor of interleukin 6
  • TCF5NFIL6
  • Transcription factor 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
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Western Blot: CEBP alpha Antibody (5B7) [NBP2-37497] - Western blot analysis using CEBPA mouse mAb against Jurkat (1), k562 (2), and HepG2 (3) cell lysate.
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Immunocytochemistry/Immunofluorescence: JunD [p Ser255] Antibody (JJ08-21) [NBP2-67861] - Staining Phospho-JunD(S255) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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CEBP Beta Antibody
(SC55-05)
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CEBP Beta Antibody (SC55-05)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IP

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Bioinformatics

Gene Symbol CEBPB