CEACAM4 Antibody (568410) [Unconjugated]

Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, IMR-90 human lung fibroblast cell line, NCI-H596 human lung adenosquamous carcinoma cell line, and human lung cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB4354) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CEACAM-4 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CEACAM-4 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human CEACAM-4 Monoclonal Antibody (Catalog # MAB4354) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 568410
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CEACAM4 Antibody (568410) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CEACAM-4
  Phe36-Val140
  Accession # O75871
• Specificity: Detects human CEACAM‑4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CEACAM-1, -3, -5, -6, -7, or -8 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM4 Antibody (568410) [Unconjugated]

• carcinoembryonic antigen CGM7
• carcinoembryonic antigen gene family member 7
• carcinoembryonic antigen-related cell adhesion molecule 4
• CEACAM4
• CEACAM-4
• CGM7
• CGM7_HUMAN

Background

Carcinoembryonic antigen cell adhesion molecule 4 (CEACAM-4), also known as CGM7 and W236, is a 40 kDa transmembrane glycoprotein member of the CEACAM family of proteins. Its 209 amino acid extracellular domain contains one Ig-like V-type domain. CEACAM-4 is expressed on granulocytes as well as on B and T cell lymphoblastic leukemias. Mouse and rat orthologs of CEACAM-4 have not been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM4
• Uniprot:
  • O75871()