CEACAM1/CD66a Antibody (CC1) - BSA Free

Immunoprecipitation: CEACAM1/CD66a Antibody (CC1) [NBP1-43390] - CEACAM1 interacts with Syk. Immunoblot analysis of CEACAM1, Syk, and GAPDH in mouse B-lymphocyte from whole cell lysate and after immunoprecipitation with or without anti-CEACAM1 antibody (mAb, CC1). Data are representative of 3 independent experiments. Image collected and cropped by CiteAb from the following publication (nature.com/articles/ncomms7217) licensed under a CC-BY license.

• Reactivity: Mu
• Applications: WB, Flow, Func, ICC/IF, IHC, IP, B/N
• Clone: CC1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 5 mg/ml

CEACAM1/CD66a Antibody (CC1) - BSA Free Summary

• Immunogen: The immunogen for this antibody was CD66a.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • In vitro assay
  • Western Blot
• Application Notes: This CC1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. Use in Immunohistochemistry-Frozen, Immunoprecipitation, western blot, In vitro assay, blocking / neutralizing, and FLOW reported in scientific literature (PMID 25692415). Use in ICC/IF was reported in scientific literature (PMID: 25692415).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CEACAM1/CD66a Antibody (CC1) - BSA Free

• antigen CD66
• BGP1
• BGP-1
• BGP1BGPBiliary glycoprotein 1
• BGPI
• Biliary Glycoprotein 1
• biliary glycoprotein adhesion molecule
• carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein)
• carcinoembryonic antigen-related cell adhesion molecule 1
• CD66a antigen
• CD66a
• Cea-1
• CEACAM1
• CEACAM-1
• Hv-1
• Hv-2
• MHVR

Background

CEACAM1 (BGP) belongs to the carcinoembryonic antigen (CEA) gene family. It is expressed on cells of epithelial and myeloid origin. CEACAM1 expression is downregulated in many tumors indicating a tumor - suppressive function. The antitumor effect may be due to inhibition of tumor angiogenesis, possibly by increased secretion of antiangiogenic molecules from the cells. Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A (A1, A2) and one B domain. While the N, A1 and B domains can also be found in other CEA-family members, the A2 domain of CEACAM1 differs from those found in other CEACAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEACAM1/CD66a Antibody (NBP1-43390)(1)

Publication using NBP1-43390

• Khairnar V, Duhan V, Maney SK et al. CEACAM1 induces B-cell survival and is essential for protective antiviral antibody production Nat Commun. 2015-02-19 [PMID: 25692415] (In vitro, IP, WB, FLOW, B/N, IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: CEACAM1
• Entrez:
  • 1048(Human)
• Uniprot:
  • P06731()