Recombinant Rat CDNF Protein

SDS-Page: Rat CDNF Protein [NBP2-35246]

• Reactivity: Rt
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rat CDNF Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 163 amino acids corresponding to CDNF Source: E. coli

  Uniprot ID: P0C5I0

  Amino Acid Sequence: QGLEAGVRSR ADCEVCKEFL NRFYNSLLTR GIDFSVDTIE EELISFCADT KGKENRLCYY LGATKDSATK ILGEVTRPMS VHMPTVKICE KLKKMDSQIC ELKYEKKLDL ESVDLWKMRV AELKQILHSW GEECRACAEK HDYVNLIKEL APKYVETRPQ TEL

• Details of Functionality: CDNF protein is fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 25 ug/mL on a nitrocellulose-coated microplate
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CDNF
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of CDNF as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 18.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rat CDNF Protein

• arginine-rich, mutated in early stage tumors-like 1
• ARMETL1
• ARMET-like protein 1
• CDNF
• cerebral dopamine neurotrophic factor
• Conserved dopamine neurotrophic factor

Background

Cerebral dopamine neurotrophic factor (CDNF), also known as ARMET-like protein 1, is a protein encoded by the CDNF gene and it is widely expressed in neuronal and non-neuronal tissues. The cerebral dopamine neurotrophic factor (CDNF) also is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). By research, CDNF prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons and it might be beneficial for the treatment of parkinson's disease. Recombinant rat CDNF contains 163 amino acid residues and it shares 83 % and 87 % a.a. sequence identity with human and murine CDNF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CDNF