Recombinant Human CDNF Protein

SDS-Page: Human CDNF Protein [NBP2-34938]

Product Discontinued

Recombinant Human CDNF Protein Summary

• Description: A single, non-glycosylated biologically active polypeptide chain corresponding to 161 residues of CDNF.
  
  

  Source: E. coli

  Amino Acid Sequence: QEAGGRPGAD CEVCKEFLNR FYKSLIDRGV NFSLDTIEKE LISFCLDTKG KENRLCYYLG ATKDAATKIL SEVTRPMSVH MPAMKICEKL KKLDSQICEL KYEKTLDLAS VDLRKMRVAE LKQILHSWGE ECRACAEKTD YVNLIQELAP KYAATHPKTE L

• Details of Functionality: CDNF protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 25 ug/ml, corresponding to a specific activity of > 40 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CDNF
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 18.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 10 mM PB, 150 mM NaCl, pH 6.4.
• Concentration: LYOPH
• Purity: >95% pure by SDS-PAGE
• Reconstitution Instructions: Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Alternate Names for Recombinant Human CDNF Protein

• arginine-rich, mutated in early stage tumors-like 1
• ARMETL1
• ARMET-like protein 1
• CDNF
• cerebral dopamine neurotrophic factor
• Conserved dopamine neurotrophic factor

Background

Cerebral dopamine neurotrophic factor (CDNF), also known as ARMET-like protein 1, is encoded by the CDNF gene in humans and widely expressed in neuronal and non-neuronal tissues. The CDNF is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons comparable to that of glial cell line-derived neurotrophic factor (GDNF). Furthermore, it also prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. By research, CDNF might be beneficial for the treatment of Parkinson's disease. Recombinant human CDNF contains 162 amino acid residues and it shares 81 % and 84 % a.a. sequence identity with murine and rat CDNF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CDNF