CDKAL1 Antibody - BSA Free

Western Blot: CDKAL1 Antibody [NBP2-98668] - Anti-CDKAL1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lane D: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 65 kDa

Immunoprecipitation: CDKAL1 Antibody [NBP2-98668] - CDKAL1 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-CDKAL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CDKAL1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 65 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CDKAL1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human CDKAL1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDKAL1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CDKAL1 Antibody - BSA Free

• CDK5 regulatory subunit associated protein 1-like 1
• CDK5 regulatory subunit-associated protein 1-like 1
• FLJ20342
• FLJ46705
• MGC75469

Background

CDKAL1 is a gene that codes for a protein with three isoforms, with lengths of 579, 488, and 97 amino acids and weights of approximately 65, 55, and 11 kDa respectively. Current studies are being done on several diseases and disorders linked to this gene including polycystic ovary syndrome, spondylitis, arterial calcification, inflammatory bowel disease, gestational diabetes, ulcerative colitis, insulin resistance, Crohn's disease, psoriasis, prostate cancer, atherosclerosis, obesity, prostatitis, pancreatitis, and malaria. CDKAL1 has also been shown to have interactions with GINS4, ATG2A, UBC, and ELAVL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDKAL1