Cdk5 Antibody (SR39-01)

Western Blot: Cdk5 Antibody (SR39-01) [NBP2-67319] - Western blot analysis of Cdk5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb

Immunohistochemistry-Paraffin: Cdk5 Antibody (SR39-01) [NBP2-67319] - Analysis of paraffin-embedded rat brain tissue using anti-Cdk5 antibody. Counter stained with hematoxylin.

Flow Cytometry: Cdk5 Antibody (SR39-01) [NBP2-67319] - Analysis of Hela cells with Cdk5 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Cdk5 Antibody (SR39-01) [NBP2-67319] - Analysis of Cdk5 on Hela cell lysates using anti-Cdk5 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Cdk5 Antibody (SR39-01) [NBP2-67319] - Analysis of paraffin-embedded mouse brain tissue using anti-Cdk5 antibody. Counter stained with hematoxylin.

Product Discontinued

Cdk5 Antibody (SR39-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Cdk5 aa 243-292 / 292. (SwissProt: Q00535 Human; SwissProt: P49615 Mouse; SwissProt: Q03114 Rat)
• Localization: Cytoplasm, Nucleus, Cell membrane, Perikaryon, Cell junction
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDK5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Application Notes: IP: Use at an assay dependent concentration.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cdk5 Antibody (SR39-01)

• CDK5
• CDKN5
• Cell division protein kinase 5
• cyclin-dependent kinase 5
• EC 2.7.11
• EC 2.7.11.22
• LIS7
• protein kinase CDK5 splicing
• PSSALRE
• Serine/threonine-protein kinase PSSALRE
• Tau protein kinase II catalytic subunit
• TPKII catalytic subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK5