CDK5 Activator 1 Antibody

Product Discontinued

CDK5 Activator 1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 1-54 of Human p35.
• Specificity: CDK5R1 antibody detects endogenous levels of CDK5R1-p35 protein, and does not cross-react with CDK5R1-p25 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK5R1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CDK5 Activator 1 Overexpression Lysate
  Human Brain Cerebellum Whole Tissue Lysate (Adult Whole Progressive Supranuclear Palsy)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDK5 Activator 1 Antibody

• CDK5 Activator 1
• CDK5P35
• CDK5R
• CDK5R1
• cyclin-dependent kinase 5 activator 1
• Cyclin-dependent kinase 5 regulatory subunit 1
• cyclin-dependent kinase 5, regulatory subunit 1 (p35)
• MGC33831
• NCK5A
• Neuronal CDK5 Activator
• p23
• p25
• p35
• p35nck5a
• regulatory partner for CDK5 kinase
• tau protein kinase II 23kDa subunit
• TPKII Regulatory Subunit

Background

Cyclin dependent kinase-5 (Cdk5), a key regulator of cell cycle progression, was originally isolated on the basis of its structural homology to Cdc2, a wellcharacterized regulator of cell cycle progression. Although Cdk5 is expressed at the highest level in the brain of adult mice, intermediate levels in testis and low or undetectable levels in all other tissues, brain is the only tissue from which Cdk5 can be isolated as an active kinase. These findings may be explained by the cloning and characterization of a Cdk5 regulatory subunit, designated p35. p35 displays a neuronal cell-specific pattern of expression, physically associates with Cdk5 and activates Cdk5 enzymatic activity. p35 is also expressed in many tissues in a truncated form, designated p25.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDK5 Activator 1 Antibody (NB100-92383)(1)

Publication using NB100-92383

• SavY A, kaikkonen MU, Leger A et al. Genetics instability of wtAAV2 genome and AAV promoter activities in the Baculovirus/Sf9 cells system. PLoS ONE. 2018-07-05 [PMID: 29975713] (WB)

Bioinformatics

• Gene Symbol: CDK5R1
• Entrez:
  • 8851(Human)
  • 116671(Rat)