CDK2 [p Thr160] Antibody (ARC57166)

Western Blot: CDK2 [p Thr160] Antibody (ARC57166) [NBP3-33582] - Western Blot analysis of lysates from C6 cells using Phospho-CDK2-T160 Rabbit mAb at 1:1000 dilution. C6 cells were treated by Nocodazole (10ng/ml).Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

Western Blot: CDK2 [p Thr160] Antibody (ARC57166) [NBP3-33582] - Western Blot analysis of lysates from HeLa cells using Phospho-CDK2-T160 Rabbit mAb at 1:1000 dilution. HeLa cells were treated by Nocodazole (20ng/ml).Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.

• Reactivity: Hu, Rt
• Applications: WB, ELISA
• Clone: ARC57166
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CDK2 [p Thr160] Antibody (ARC57166) Summary

• Description: Novus Biologicals Rabbit CDK2 [p Thr160] Antibody (ARC57166) (NBP3-33582) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic phosphorylated peptide around T160 of human CDK2 (NP_001789.2).
  
  Sequence:
  TYTHE
• Modification: p Thr160
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDK2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for CDK2 [p Thr160] Antibody (ARC57166)

• cdc2-related protein kinase
• CDK2
• cell devision kinase 2
• Cell division protein kinase 2
• cyclin-dependent kinase 2
• EC 2.7.11
• EC 2.7.11.22
• p33 protein kinase
• p33(CDK2)

Background

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK2