CDK2 Antibody (1A6)

Images

 
Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Western blot analysis using CDK2 mouse mAb against Jurkat (1), HL-60 (2), K562(3), A431(4), HeLa(5), and NIH3T3 (6) cell lysate.
Immunocytochemistry/ Immunofluorescence: CDK2 Antibody (1A6) [NBP2-37507] - Immunofluorescence analysis of HeLa cells using CDK2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: CDK2 Antibody (1A6) [NBP2-37507] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CDK2 mouse mAb with DAB staining.
Flow Cytometry: CDK2 Antibody (1A6) [NBP2-37507] - Flow cytometric analysis of Jurkat cells using CDK2 mouse mAb (green) and negative control (red).
Western Blot: CDK2 Antibody (1A6) [NBP2-37507] - Western blot analysis using CDK2 mAb against human CDK2 (AA: 197-295) recombinant protein. (Expected MW is 36.8 kDa)
Immunohistochemistry: CDK2 Antibody (1A6) [NBP2-37507] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CDK2 mouse mAb with DAB staining.
Immunohistochemistry-Paraffin: CDK2 Antibody (1A6) [NBP2-37507] - analysis of CDK2 in paraffin embedded human colon cancer tissue using anti-CDK2 antibody. Image from verified customer review.
ELISA: CDK2 Antibody (1A6) [NBP2-37507] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Clone
1A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CDK2 Antibody (1A6) Summary

Immunogen
Purified recombinant fragment of human CDK2 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDK2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
Theoretical MW
33.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CDK2 Lysate (NBP2-65935)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-37507 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CDK2 Antibody (1A6)

  • cdc2-related protein kinase
  • CDK2
  • cell devision kinase 2
  • Cell division protein kinase 2
  • cyclin-dependent kinase 2
  • EC 2.7.11
  • EC 2.7.11.22
  • p33 protein kinase
  • p33(CDK2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDK2 Antibody (NBP2-37507) (0)

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Review for CDK2 Antibody (NBP2-37507) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-37507:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CDK2 NBP2-37507
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reviewed by:
Anonymous
IHC-P Human 01/08/2016
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedColon cancer
SpeciesHuman
Lot140523

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CDK2 Antibody (NBP2-37507) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CDK2 Antibody (NBP2-37507)

Discover related pathways, diseases and genes to CDK2 Antibody (NBP2-37507). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CDK2.

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EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

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Recent Reviews

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1
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Anonymous
01/08/2016
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CDK2
Entrez