CDCP1 Antibody

Images

 
Western Blot: CDCP1 Antibody [NB100-860] - (1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer).
Immunohistochemistry-Paraffin: CDCP1 Antibody [NB100-860] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CDCP1 Antibody Summary

Immunogen
Peptide with sequence C-PLLNTQEPMEPAE corresponding to C-Terminus according to NP_073753.3.
Specificity
This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDCP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
IP successful usage with A549 cells. WB: Approx. 90 kDa band observed in human colon lysates (calculated MW of 92.3 kDa band according to NP_073753.3). An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC successful usage for human endometrium.
IHC: Paraffin embedded Human Prostate.
Positive Control
CDCP1 Lysate (NBL1-09023)
Publications
Read Publications using NB100-860.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDCP1 Antibody

  • CD318 antigen
  • CD318
  • CDCP1
  • CUB domain containing protein 1
  • CUB domain-containing protein 1
  • Membrane glycoprotein gp140
  • SIMA135
  • SIMA135TRASK
  • Subtractive immunization M plus HEp3-associated 135 kDa protein
  • Transmembrane and associated with src kinases

Background

The protein encoded by this gene is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDCP1 Antibody (NB100-860)(2)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDCP1
Entrez
Uniprot