CDCP1 Antibody

Western Blot: CDCP1 Antibody [NB100-860] - (1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer).

Immunohistochemistry-Paraffin: CDCP1 Antibody [NB100-860] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CDCP1 Antibody Summary

• Description: Novus Biologicals Goat CDCP1 Antibody (NB100-860) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-CDCP1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PLLNTQEPMEPAE corresponding to C-Terminus according to NP_073753.3.
• Specificity: This antibody is expected to recognize isoform 1 (NP_073753.3) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDCP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: IP successful usage with A549 cells. WB: Approx. 90 kDa band observed in human colon lysates (calculated MW of 92.3 kDa band according to NP_073753.3). An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC successful usage for human endometrium.
  IHC: Paraffin embedded Human Prostate.
• Control:

  CDCP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDCP1 Antibody

• CD318 antigen
• CD318
• CDCP1
• CUB domain containing protein 1
• CUB domain-containing protein 1
• Membrane glycoprotein gp140
• SIMA135
• SIMA135TRASK
• Subtractive immunization M plus HEp3-associated 135 kDa protein
• Transmembrane and associated with src kinases

Background

The protein encoded by this gene is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDCP1 Antibody (NB100-860)(2)

Publications using NB100-860

• Sasaroli D, Gimotty PA, Pathak HB et al. Novel surface targets and serum biomarkers from the ovarian cancer vasculature. Cancer Biol Ther. 2011-08-01 [PMID: 21617380]
• Hooper JD, Zijlstra A, Aimes RT et al. Subtractive immunization using highly metastatic human tumor cells identifiesSIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene 2003-03-27 [PMID: 12660814]

Bioinformatics

• Gene Symbol: CDCP1
• Entrez:
  • 64866(Human)
• Uniprot:
  • NP_073753.(Human)