CDCP1 Overexpression Lysate

Images

 
Western Blot: CDCP1 Overexpression Lysate (Adult Normal) [NBL1-09023] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDCP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CDCP1 Overexpression Lysate Summary

Description

CDCP1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC220633

Accession#: NM_022842

Protein Tag: C-MYC/DDK
Gene
CDCP1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
92.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CDCP1 Overexpression Lysate

  • CD318 antigen
  • CD318
  • CDCP1
  • CUB domain containing protein 1
  • CUB domain-containing protein 1
  • Membrane glycoprotein gp140
  • SIMA135
  • SIMA135TRASK
  • Subtractive immunization M plus HEp3-associated 135 kDa protein
  • Transmembrane and associated with src kinases

Background

The protein encoded by this gene is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CDCP1 Lysate (NBL1-09023) (0)

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Video Protocols

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Bioinformatics

Gene Symbol CDCP1