CDCP1 Antibody [Unconjugated]

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the apices of colonic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the apices of colonic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="CDCP1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2666) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the apices of colonic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

CDCP1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Goat Anti-Human CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2666) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " > AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " title="SW480 cells were stained with Goat Anti-Human CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2666, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins. " />

SW480 cells were stained with Goat Anti-Human CDCP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2666, filled histogram) or isotype control antibody (Catalog # <a class=NoLineLink href=

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CDCP1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CDCP1 isoform 1
  Phe30-Leu666
  Accession # NP_073753
• Specificity: Detects human CDCP1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDCP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDCP1 Antibody [Unconjugated]

• CD318 antigen
• CD318
• CDCP1
• CUB domain containing protein 1
• CUB domain-containing protein 1
• Membrane glycoprotein gp140
• SIMA135
• SIMA135TRASK
• Subtractive immunization M plus HEp3-associated 135 kDa protein
• Transmembrane and associated with src kinases

Background

CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Sre kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDCP1 Antibody (AF2666)(5)

Publications using AF2666

• T Kryza, T Khan, S Puttick, C Li, KA Sokolowski, BW Tse, T Cuda, N Lyons, M Gough, J Yin, A Parkin, EI Deryugina, JP Quigley, RHP Law, JC Whisstock, AD Riddell, AP Barbour, DK Wyld, PA Thomas, S Rose, CE Snell, M Pajic, Y He, JD Hooper Effective targeting of intact and proteolysed CDCP1 for imaging and treatment of pancreatic ductal adenocarcinoma Theranostics, 2020-03-04;10(9):4116-4133. 2020-03-04 [PMID: 32226543] (Western Blot, Human)
• Yang Chen, Brittney S. Harrington, Kevin C.N. Lau, Lez J. Burke, Yaowu He, Mary Iconomou et al. Development of an enzyme-linked immunosorbent assay for detection of CDCP1 shed from the cell surface and present in colorectal cancer serum specimens Journal of Pharmaceutical and Biomedical Analysis 2017-05-30 [PMID: 28279929] (Western Blot, Human)
• Lifang Yang, Sucharita M. Dutta, Dean A. Troyer, Jefferson B. Lin, Raymond A. Lance, Julius O. Nyalwidhe et al. Dysregulated expression of cell surface glycoprotein CDCP1 in prostate cancer Oncotarget 2015-12-22 [PMID: 26497208]
• Kelly OG, Chan MY, Martinson LA Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat. Biotechnol., 2011-07-31;29(8):750-6. 2011-07-31 [PMID: 21804561] (IHC-Fr, Human)
• He Y, Wortmann A, Burke LJ Proteolysis-induced N-terminal ectodomain shedding of the integral membrane glycoprotein CUB domain-containing protein 1 (CDCP1) is accompanied by tyrosine phosphorylation of its C-terminal domain and recruitment of Src and PKCdelta. J. Biol. Chem., 2010-06-15;285(34):26162-73. 2010-06-15 [PMID: 20551327] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CDCP1
• Uniprot:
  • NP_073753()