CDCP1 Antibody (OTI2C1) - Azide and BSA Free

Western Blot: CDCP1 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72156] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCP1.

Immunocytochemistry/Immunofluorescence: CDCP1 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72156] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CDCP1.

Immunohistochemistry: CDCP1 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72156] - Staining of paraffin-embedded Human lymphoma tissue using anti-CDCP1 mouse monoclonal antibody.

Flow Cytometry: CDCP1 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72156] - Analysis of Jurkat cells, using anti-CDCP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry: CDCP1 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72156] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CDCP1 mouse monoclonal antibody.

Immunohistochemistry: CDCP1 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72156] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDCP1 mouse monoclonal antibody.

Immunohistochemistry: CDCP1 Antibody (OTI2C1) - Azide and BSA Free [NBP2-72156] - Staining of paraffin-embedded Human prostate tissue using anti-CDCP1 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI2C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CDCP1 Antibody (OTI2C1) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human CDCP1 (NP_073753) produced in HEK293T cell.
• Specificity: This antibody also reacts with SNX8 and TRAF1
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDCP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-500
• Theoretical MW: 92.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for CDCP1 Antibody (OTI2C1) - Azide and BSA Free

• CD318 antigen
• CD318
• CDCP1
• CUB domain containing protein 1
• CUB domain-containing protein 1
• Membrane glycoprotein gp140
• SIMA135
• SIMA135TRASK
• Subtractive immunization M plus HEp3-associated 135 kDa protein
• Transmembrane and associated with src kinases

Background

CDCP1 is encoded by this gene is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDCP1