CDC37 [p Ser13] Antibody (SR1889) - BSA Free

Western Blot: CDC37 [p Ser 13] Antibody (SR1889) [NBP3-21571] - Western blot analysis using NBP3-21571 in (1) Jurkat cell lysate treated with Alkaline Phosphatase; (2) Jurkat cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clone: SR1889
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CDC37 [p Ser13] Antibody (SR1889) - BSA Free Summary

• Description: Novus Biologicals Rabbit CDC37 [p Ser13] Antibody (SR1889) - BSA Free (NBP3-21571) is a recombinant monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-CDC37 [Ser13] (Uniprot #: Q16543)
• Modification: p Ser13
• Specificity: Detects endogenous levels of total phospho-CDC37 [Ser13]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC37
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CDC37 [p Ser13] Antibody (SR1889) - BSA Free

• CDC37 (cell division cycle 37, S. cerevisiae, homolog)
• CDC37 cell division cycle 37 homolog (S. cerevisiae)
• CDC37 cell division cycle 37 homolog
• CDC37A
• cell division cycle 37 homolog (S. cerevisiae)
• Hsp90 chaperone protein kinase-targeting subunit
• hsp90 co-chaperone Cdc37
• P50CDC37

Background

In S. cerevisiae, Cdc37 has been shown to be an important regulator of cell division cycle through its action on cyclin-dependent kinases (CDK's). Cdc37 has also been associated with signaling pathways and kinases unrelated to cell cycle control, including v-Src, casein kinase II, MPS1 kinase and the sevenless receptor tyrosine kinase. Interestingly, HSP90 has also been shown to be associated with many of the same cellular targets as Cdc37. It has now been shown that Cdc37 is the same protein previously described as p50 which has been found to be associated with many of the same proteins as HSP90. Among others, Cdc37/p50 has been found complexed with Src-family kinases (Fyn, Yes, Fes, Lck & Fgr), Raf and pp60v-src. More recently, Cdc37 has been shown to be a chaperone independently and in concert with HSP90. In fact, Cdc37 is necessary for the binding of HSP90 to Cdk4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC37