Cdc20 Antibody (AR12) [PE/Cy7]

Product Discontinued

Cdc20 Antibody (AR12) [PE/Cy7] Summary

• Immunogen: Urea-denatured His6 Cdc20 human recombinant protein (Uniprot: Q12834)
• Localization: Cytoplasmic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC20
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for Cdc20 Antibody (AR12) [PE/Cy7]

• CDC20 cell division cycle 20 homolog (S. cerevisiae)
• CDC20 cell division cycle 20 homolog
• Cdc20
• cell division cycle 20 homolog (S. cerevisiae)
• cell division cycle protein 20 homolog
• MGC102824
• p55CDC
• p55CDCS. cerevisiae, homolog)

Background

CDC20 is a WD repeat containing cell cycle-regulator that binds to and activates the ubiquitin-ligase activity of the APC/c (anaphase-promoting complex/cyclosome). APC/c ubiquitinating activity is responsible for the degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. CDC20 has also been reported to form a complex with histone deacetylases and can function as a transcriptional repressor involved in cell cycle arrest. As a regulator of APC/c activity, CDC20 plays a key role in the spindle-assembly checkpoint.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC20