CD74 Antibody (LN-2)


Immunohistochemistry-Frozen: CD74 Antibody (LN-2) [NBP2-29465] - Analysis using the Azide and BSA Free version of NBP2-29465. Staining of human tonsil stained with CD74 MAb (LN-2).

Product Details

Reactivity Hu, Mu, Bb, Rt(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Please see the vial label for concentration. If unlisted please contact technical services.

CD74 Antibody (LN-2) Summary

SU-DHL-4 lymphoma cells were used as the immunogen for the CD74 antibody
Cell surface and paranuclear globular
B-Cell Marker
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). This antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells.
IgG1 Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS with 0.05% BSA.
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein G purified


Application Notes
Human Chromosome Location: 5q33.1
Use in Western Blot has been reported in literature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
37 kDa
Read Publication using NBP2-29465.

Alternate Names for CD74 Antibody (LN-2)

  • CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
  • CD74 antigen
  • CD74 molecule, major histocompatibility complex, class II invariant chain
  • CD74
  • CLIP
  • DHLAGgamma chain of class II antigens
  • HLA class II histocompatibility antigen gamma chain
  • HLA-DR antigens-associated invariant chain
  • HLA-DR-gamma
  • Ia antigen-associated invariant chain
  • Ia-associated invariant chain
  • Ia-gamma
  • Ii
  • INVG34
  • MHC HLA-DR gamma chain
  • p33


CD74, a Type-II transmembrane protein, is a unique member of RIP-processed protein family. It consists of an extracytoplasmic domain, a transmembrane segment and an intracytoplasmic domain. It is expressed in B-cells, monocytes, macrophages and other Class II MHC expressing cells. CD74 associates with alpha and beta chains of HLA-DR (MHC class II) and directs intracellular sorting of MHC class II molecules. It has a prominent role in antigen presentation and as an accessory signaling molecule. Cell-surface CD74 binds to MIF and its expression is required for MIF-mediated ERK1/2-phosphorylation, PGE2 production and cell proliferation. CD74 regulates B-cell differentiation and acts as a survival receptor through activation of NF-kB p65/RelA homodimer. Pathological role of CD74 has been identified in different cancers including Multiple Myeloma.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD74 Antibody (NBP2-29465)(1)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Blogs on CD74.

CD74 - a central player in antigen presentation by MHC class II
Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in...  Read full blog post.

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Gene Symbol CD74

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