CD74 Antibody (829706) [DyLight 650]

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 829706
• Clonality: Monoclonal
• Host: Rat
• Conjugate: DyLight 650

CD74 Antibody (829706) [DyLight 650] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD74
  Gln56-Cys215
  Accession # P04441
• Specificity: Detects mouse CD74 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD74 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CD74
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CD74 Antibody (829706) [DyLight 650]

• CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
• CD74 antigen
• CD74 molecule, major histocompatibility complex, class II invariant chain
• CD74
• CLIP
• DHLAG
• DHLAGgamma chain of class II antigens
• HLA class II histocompatibility antigen gamma chain
• HLADG
• HLA-DR antigens-associated invariant chain
• HLA-DR-gamma
• Ia antigen-associated invariant chain
• Ia-associated invariant chain
• Ia-gamma
• Ii
• INVG34
• MHC HLA-DR gamma chain
• p33

Background

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of, or in association with, MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41 kDa, depending on genetic splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD74 Antibody (FAB7478W)(1)

Publication using FAB7478W

• Cantalupo P, Diacou A, Park S, Soman V et Al. Single-cell RNA-seq reveals a resolving immune phenotype in the oral mucosa iScience 2024-09-16 [PMID: 39280609]

Bioinformatics

• Gene Symbol: CD74