CD74 Antibody (829706) [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: Flow
• Clone: 829706
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 488
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

CD74 Antibody (829706) [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse CD74
  Gln56-Cys215
  Accession # P04441
• Specificity: Detects mouse CD74 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD74 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CD74
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD74 Antibody (829706) [Alexa Fluor® 488]

• CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
• CD74 antigen
• CD74 molecule, major histocompatibility complex, class II invariant chain
• CD74
• CLIP
• DHLAG
• DHLAGgamma chain of class II antigens
• HLA class II histocompatibility antigen gamma chain
• HLADG
• HLA-DR antigens-associated invariant chain
• HLA-DR-gamma
• Ia antigen-associated invariant chain
• Ia-associated invariant chain
• Ia-gamma
• Ii
• INVG34
• MHC HLA-DR gamma chain
• p33

Background

CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature mouse CD74 consists of a 29 amino acid (aa) cytoplasmic domain, a 29 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2). Alternative splicing generates a short isoform that lacks the thyroglobulin domain (2). Within the ECD, mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (3). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (4, 5). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up‑regulation of angiogenic factors in endometrial stromal cells (6‑11). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (10). CD74 is upregulated on non‑immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (12, 13). It is also upregulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 14).

1. Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
2. Koch, N. et al. (1987) EMBO J. 6:1677.
3. Riberdy, J.M. et al. (1992) Nature 360:474.
4. Koch, N. et al. (1991) J. Immunol. 147:2643.
5. Roche, P.A. et al. (1991) Nature 354:392.
6. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
7. Takahashi, K. et al. (2009) Respir. Res. 10:33.
8. Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. USA 104:17444.
9. Shi, X. et al. (2006) Immunity 25:595.
10. Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
11. Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
12. Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
13. Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
14. Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD74 Antibody (FAB7478G)(1)

Publication using FAB7478G

• K David, G Friedlande, B Pellegrino, L Radomir, H Lewinsky, L Leng, R Bucala, S Becker-Her, I Shachar CD74 as a regulator of transcription in normal B cells Cell Reports, 2022-11-01;41(5):111572. 2022-11-01 [PMID: 36323260] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CD74
• Uniprot:
  • P04441()