CD72 Antibody [Unconjugated]

CD72 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CD72 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD72 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD72
  Arg117-Asn354
  Accession # P21855
• Specificity: Detects mouse CD72 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CD72 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD72
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD72 Antibody [Unconjugated]

• CD72 antigenB-cell differentiation antigen CD72
• CD72 molecule
• CD72
• CD72b
• Ly-19.2
• Ly-32.2
• Lyb-2
• LYB2lyb-2

Background

CD72, also named B-cell differentiation antigen Lyb-2, is a Type II transmembrane protein with a C-type lectin domain. It associates with CD5 and is a functional receptor for Semaphorin 4D in the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD72 Antibody (AF1279)(1)

Publication using AF1279

• Iratxe Zuazo-Gaztelu, Marta Pàez-Ribes, Patricia Carrasco, Laura Martín, Adriana Soler, Mar Martínez-Lozano et al. Anti-tumor effects of anti-Semaphorin 4D antibody unravel a novel pro-invasive mechanism of vascular targeting agents Cancer Research 2019-10-15 [PMID: 31239269]

Bioinformatics

• Gene Symbol: CD72
• Uniprot:
  • P21855()