CD6 Antibody (3F7B5)

Images

 
Immunocytochemistry/ Immunofluorescence: CD6 Antibody (3F7B5) [NBP2-32942] - Immunofluorescence staining of MOLT-4 cells using CD6 Antibody (3F7B5) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are ...read more
Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD6 MAb (3F7B5).
Flow Cytometry: CD6 Antibody (3F7B5) [NBP2-32942] - Flow Cytometric Analysis of MOLT-4 cells. CD6 Antibody (3F7B5) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD6 MAb (3F7B5).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
3F7B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CD6 Antibody (3F7B5) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34537)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Additional Information
Clone 3F7B5 was used by HLDA to establish CD designation.
Immunogen
Human rheumatoid synovial T cell line ST-1
Localization
Cell surface and cytoplasmic
Marker
Negative Marker of T-regulatory Cells
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CD6 Antibody (3F7B5)

  • CD6 antigenFLJ44171
  • CD6 molecule
  • CD6
  • T12
  • Tp120
  • TP120T-cell differentiation antigen CD6

Background

CD6 is a 100-130 kD single chain transmembrane glycoprotein also known as T12. It is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6, interacting with its ligand CD166 (also known as ALCAM), is involved in T cell development and activation, as well as thymocyte adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD6 Antibody (NBP2-32942) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD6
Uniprot