CD59 Antibody [Unconjugated]

Western blot shows lysates of human breast tissue and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD59 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and HUVEC human umbilical vein endothelial cells, loaded at 0.5 mg/ml. A specific band was detected for CD59 at approximately 27-33 kDa (as indicated) using 50 µg/ml of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by 1:50 dilution of HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

CD59 was detected in immersion fixed paraffin-embedded sections of human breast using 1.7 µg/mL Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for CD59 at approximately 26 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD59 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD59
  Leu26-Asn102
  Accession # P13987
• Specificity: Detects human CD59 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD59
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD59 Antibody [Unconjugated]

• 16.3A5
• 1F5 antigen
• 1F5
• 20 kDa homologous restriction factor
• CD59 antigen
• CD59 antigen, complement regulatory protein
• CD59 glycoprotein
• CD59 molecule, complement regulatory protein
• CD59
• EJ16
• EJ30
• EJ30, EL32 and G344)
• EL32
• FLJ38134
• FLJ92039
• G344
• HRF20
• HRF-20
• human leukocyte antigen MIC11
• Ly-6-like protein
• lymphocytic antigen CD59/MEM43
• MACIF
• MAC-inhibitory protein
• MAC-IP
• MEM43 antigen
• MEM43
• membrane attack complex (MAC) inhibition factor
• Membrane attack complex inhibition factor
• Membrane inhibitor of reactive lysis
• MGC2354
• MIC11
• MIC11MSK21
• MIN1
• MIN2
• MIN3
• MIRL
• p18-20
• Protectin
• surface anitgen recognized by monoclonal 16.3A5
• T cell-activating protein

Background

CD59 is a GPI-anchored membrane glycoprotein that functions to inhibit formation of the complement membrane attack complex and thereby prevent cell injury. CD59 is expressed on erythrocytes and on all peripheral blood leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD59 Antibody (AF1987)(4)

Publications using AF1987

• Goudsmit, C, da Veiga Leprevost, F, et al. Differences in Extracellular Vesicle Protein Cargo Are Dependent on Head and Neck Squamous Cell Carcinoma Cell of Origin and Human Papillomavirus Status. Cancers (Basel) [PMID: 34359613]
• J Fisher, OE Sørensen, AHA Abu-Humaid Using chemiluminescence imaging of cells (CLIC) for relative protein quantification Sci Rep, 2020-10-26;10(1):18280. 2020-10-26 [PMID: 33106566] (ICC, Human)
• Antonelou MH, Kriebardis AG, Stamoulis KE Apolipoprotein J/Clusterin in Human Erythrocytes Is Involved in the Molecular Process of Defected Material Disposal during Vesiculation. PLoS ONE, 2011-10-07;6(10):e26033. 2011-10-07 [PMID: 22016805] (Western Blot, Human)
• Wosik K, Cayrol R, Dodelet-Devillers A, Berthelet F, Bernard M, Moumdjian R, Bouthillier A, Reudelhuber TL, Prat A Angiotensin II controls occludin function and is required for blood brain barrier maintenance: relevance to multiple sclerosis. J. Neurosci., 2007-08-22;27(34):9032-42. 2007-08-22 [PMID: 17715340] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CD59
• Uniprot:
  • P13987()