CD59 Antibody (193-27) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CD59 Antibody (193-27) - Chimeric [NBP3-12078] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 193-27 (NBP3-12078) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12078, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: CD59 Antibody (193-27) - Chimeric - Azide and BSA Free [NBP3-12078] - Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12078 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC
• Clone: 193-27
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD59 Antibody (193-27) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD59 Antibody (193-27) - Chimeric - Azide and BSA Free (NBP3-12078) is a recombinant monoclonal antibody validated for use in IHC, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunizng mice with stimulated human leukocytes.
• Specificity: This antibody recognizes human CD59 which is a glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. It regulates complement-mediated cell lysis, and is involved in lymphocyte signal transduction.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD59
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD59 Antibody (193-27) - Chimeric - Azide and BSA Free

• 16.3A5
• 1F5 antigen
• 1F5
• 20 kDa homologous restriction factor
• CD59 antigen
• CD59 antigen, complement regulatory protein
• CD59 glycoprotein
• CD59 molecule, complement regulatory protein
• CD59
• EJ16
• EJ30
• EJ30, EL32 and G344)
• EL32
• FLJ38134
• FLJ92039
• G344
• HRF20
• HRF-20
• human leukocyte antigen MIC11
• Ly-6-like protein
• lymphocytic antigen CD59/MEM43
• MACIF
• MAC-inhibitory protein
• MAC-IP
• MEM43 antigen
• MEM43
• membrane attack complex (MAC) inhibition factor
• Membrane attack complex inhibition factor
• Membrane inhibitor of reactive lysis
• MGC2354
• MIC11
• MIC11MSK21
• MIN1
• MIN2
• MIN3
• MIRL
• p18-20
• Protectin
• surface anitgen recognized by monoclonal 16.3A5
• T cell-activating protein

Background

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD59