CD52 Antibody (HI186) - BSA Free

Images

 
Flow Cytometry: CD52 Antibody (HI186) [NB500-311] - Flow cytometry analysis of CD52 in patients suffering with Acute Lymphoblastic Leukemia (anti-human CD52 (HI186); detection by Goat anti-mouse IgG2b PE). Fig. 1A CD52 ...read more
Flow Cytometry: CD52 Antibody (HI186) [NB500-311] - Fig.1B CD52 negative pro T ALL (gate leukemic cells)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
HI186
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD52 Antibody (HI186) - BSA Free Summary

Additional Information
Clone HI186 was used by HLDA to establish CD designation.
Immunogen
Human tonsil (NM_001803.2).
Specificity
The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract. HLDA VI; WS Code BP 523 HLDA VI; WS Code T 6T-057
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD52
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
Purity is > 95% (by SDS-PAGE). This antibody is CyTOF ready.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD52 Antibody (HI186) - BSA Free

  • Cambridge pathology 1 antigen
  • CAMPATH-1 antigen
  • CAMPATH-1
  • CD52 antigen (CAMPATH-1 antigen)
  • CD52 antigen
  • CD52 molecule
  • CD52
  • CDW52 antigen (CAMPATH-1 antigen)
  • CDw52
  • Epididymal secretory protein E5
  • He5
  • HEL-S-171mP
  • Human epididymis-specific protein 5

Background

CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD52
Entrez
Uniprot