CD37 Antibody (424925) [Unconjugated]

Human whole blood monocytes were stained with Mouse Anti-Human CD37 Monoclonal Antibody (Catalog # MAB4625, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 424925
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD37 Antibody (424925) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD37
  Accession # Q96LM7
• Specificity: Detects human CD37. Stains human CD37 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD37
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD37 Antibody (424925) [Unconjugated]

• CD37 antigentetraspanin-26
• CD37 molecule
• CD37
• cell differentiation antigen 37
• GP52-40
• leukocyte antigen CD37
• MGC120234
• Tetraspanin-26
• TSPAN26
• Tspan-26
• TSPAN26leukocyte surface antigen CD37

Background

CD37, also known as TSPAN26, is a 40‑45 kDa palmitoylated tetraspanin superfamily glycoprotein that is expressed by lymphocytes and myeloid cells. CD37 directly associates with dectin-1 on macrophages and stabilizes cell surface dectin-1 expression. It also inhibits T cell activation and proliferation by limiting T cell receptor signaling. Human CD37 shares 80% aa sequence identity with mouse and rat CD37.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD37 Antibody (MAB4625)(1)

Publication using MAB4625

• Jorgensen MM, Baek R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles 1/1/2015 [PMID: 25862471]

Bioinformatics

• Gene Symbol: CD37
• Uniprot:
  • Q96LM7()