CD302/CLEC13A Antibody [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

CD302/CLEC13A Antibody [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD302/CLEC13A
  Asp21-His165
  Accession # Q9DCG2
• Specificity: Detects mouse CD302/CLEC13A in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human CD302 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD302/CLEC13A Antibody [Alexa Fluor® 594]

• BIMLEC
• CD302 antigenC-type lectin domain family 13, member A
• CD302 molecule
• CD302
• CLEC13A
• CLEC13AMGC22301
• C-type lectin domain family 13 member A
• DCL1
• DCL-1
• DCL1C-type lectin BIMLEC
• KIAA0022FLJ43091
• Type I transmembrane C-type lectin receptor DCL-1

Background

CD302 (also DCL-1 and CLEC13A) is a 31-36 kDa member of the C-type lectin domain family of proteins. It is expressed on cells involved in phagocytosis, including neutrophils, monocytes, dendritic cells and macrophages. CD302 is suggested to facilitate phagocytosis, and participate in cell adhesion and migration. Mature mouse CD302 is a 208 amino acid (aa) type I transmembrane glycoprotein. It contains a 146 aa extracellular domain (ECD) (aa 21-165) plus a 42 aa cytoplasmic region. The ECD possesses one C-type lectin domain (aa 30-149) that apparently cannot bind carbohydrate. In human, CD302 has also been detected in dendritic cells as the C-terminal portion of a 215 kDa fusion protein that involves DEC205. CD302 contributes aa 23-232 to this fusion protein. Mouse CD302 has two potential splice variants. One shows an alternative start site at Met61, while another shows a deletion of aa 154-162. Over aa 21-165, mouse CD302 shares 82% and 92% aa identity with human and rat CD302, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.