CD302/CLEC13A Antibody (662714) [Unconjugated]

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 2 µg/mL of Mouse CD302/CLEC13A Monoclonal Antibody (Catalog # MAB64241) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD302/CLEC13A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1

• Reactivity: Mu
• Applications: WB
• Clone: 662714
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD302/CLEC13A Antibody (662714) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD302/CLEC13A
  Met1-Cys165
  Accession # Q9DCG2
• Specificity: Detects mouse CD302/CLEC13A in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) CD302 is observed. No cross-reactivity with rhCLEC1, 2, 2A, 3B, 9A, 10A, 12B, 14A, recombinant mouse CLEC4B2, 9A, or 12A is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CD302
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD302/CLEC13A Antibody (662714) [Unconjugated]

• BIMLEC
• CD302 antigenC-type lectin domain family 13, member A
• CD302 molecule
• CD302
• CLEC13A
• CLEC13AMGC22301
• C-type lectin domain family 13 member A
• DCL1
• DCL-1
• DCL1C-type lectin BIMLEC
• KIAA0022FLJ43091
• Type I transmembrane C-type lectin receptor DCL-1

Background

CD302, also known as CLEC13A and DCL-1, is a 35 kDa type I transmembrane glycoprotein in the C-type lectin family. CD302 is expressed on monocytes, macrophages, dendritic cells, and granulocytes. It contains one carbohydrate binding domain in its extracellular region and can mediate antigen uptake. In human Hodgkin’s disease, a fusion protein containing portions of CD302 and the ECD of DEC-205 is expressed on Reed-Sternberg cells.  Within the ECD, mouse CD302 shares 82% and 92% aa sequence identity with human and rat CD302, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD302/CLEC13A Antibody (MAB64241)(1)

Publication using MAB64241

• RJP Brown, B Tegtmeyer, J Sheldon, T Khera, Anggakusum, D Todt, G Vieyres, R Weller, S Joecks, Y Zhang, S Sake, D Bankwitz, K Welsch, C Ginkel, M Engelmann, G Gerold, E Steinmann, Q Yuan, M Ott, FWR Vondran, T Krey, LJ Ströh, C Miskey, Z Ivics, V Herder, W Baumgärtne, C Lauber, M Seifert, AW Tarr, CP McClure, G Randall, Y Baktash, A Ploss, VLD Thi, E Michailidi, M Saeed, L Verhoye, P Meuleman, N Goedecke, D Wirth, CM Rice, T Pietschman Liver-expressed Cd302 and Cr1l limit hepatitis C virus cross-species transmission to mice Sci Adv, 2020-11-04;6(45):. 2020-11-04 [PMID: 33148654] (Flow Cytometry, IHC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CD302
• Uniprot:
  • Q9DCG2()