CD300LG/Nepmucin Antibody [Alexa Fluor® 700]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

CD300LG/Nepmucin Antibody [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD300g/Nepmucin
  Leu19-Ile284
  Accession # NP_001154183
• Specificity: Detects mouse CD300g/Nepmucin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human CD300g/Nepmucin is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD300LG/Nepmucin Antibody [Alexa Fluor® 700]

• CD300 antigen like family member G
• CD300 antigen-like family member G
• CD300 molecule like family member g
• CD300 molecule-like family member g
• CD300g antigen
• CD300g
• CD300LG
• CLM9
• CLM-9
• CLM9Trem4
• CMRF35-like molecule 9
• Nepmucin
• TREM4
• TREM-4
• Triggering receptor expressed on myeloid cells 4

Background

Nepmucin (a not expressed in Peyer's Patch mucin; also CLM-9, CD300g and TREM4) is a member of the CD300L family of proteins. It is expressed on endothelium and serves multiple functions. It is known to form homotypic interactions between endothelial cells, bind lymphocytes via its Ig-like and mucin domains and serve as a transcellular transport vehicle for IgA and IgM. Mature mouse Nepmucin is a 397 amino acid (aa) type I transmembrane glycoprotein. Its extracellular region (aa 19‑288) contains one V-type Ig-like domain (aa 19‑124) plus an O-glycosylated mucin region (aa 136‑276). There are at least three isoform variants. One is 60‑75 kDa in size and shows an Ala substitution for aa 126‑170, a second is 46‑50 kDa in size and shows an Arg substitution for aa 170‑254, while a third is 36 kDa in size and shows a SerTyr substitution for aa 134‑256. Over aa 1‑284, mouse Nepmucin shares 89% and 53% aa identity with rat and human Nepmucin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.