CD30 Ligand/TNFSF8 Overexpression Lysate (Native)

Images

 
Western Blot: TNFSF8 Overexpression Lysate (Adult Normal) [NBL1-17170] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TNFSF8.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CD30 Ligand/TNFSF8 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC211276 and based on accession number NM_001244. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens tumor necrosis factor (ligand) superfamily, member 8 (TNFSF8), mRNA.
Gene
TNFSF8

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
25.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CD30 Ligand/TNFSF8

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CD30 Ligand/TNFSF8 Overexpression Lysate (Native)

  • CD153 antigen
  • CD153
  • CD30 antigen ligand
  • CD30 Ligand
  • CD30L
  • CD30-L
  • CD30LCD30 ligand
  • CD30LGMGC138144
  • TNFSF8
  • tumor necrosis factor (ligand) superfamily, member 8
  • tumor necrosis factor ligand superfamily member 8

Background

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CD30 Ligand/TNFSF8 Lysate (NBL1-17170) (0)

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Bioinformatics

Gene Symbol TNFSF8
Entrez