CD27 Ligand/TNFSF7/CD70 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC200410 and based on accession number NM_001252. The protein contains a C-terminal DDK tag.
Homo sapiens CD70 molecule (CD70), mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
0.09% Sodium Azide
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Lysate Details for CD27 Ligand/TNFSF7/CD70
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for CD27 Ligand/TNFSF7/CD70 Overexpression Lysate (Native)
- CD27LCD27 ligand
- CD70 molecule
- Ki-24 antigen
- surface antigen CD70
- TNFSF7CD70 antigen
- tumor necrosis factor (ligand) superfamily, member 7
- Tumor necrosis factor ligand superfamily member 7
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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Publications for CD27 Ligand/TNFSF7/CD70 Lysate (NBL1-08965) (0)
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