CCT5 Antibody - BSA Free

Western Blot: CCT5 Antibody [NBP1-31337] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [N1N3].

Immunocytochemistry/Immunofluorescence: CCT5 Antibody [NBP1-31337] - Analysis of HeLa, using NBP1-31337 at 1:200 dilution.

Immunohistochemistry-Paraffin: CCT5 Antibody [NBP1-31337] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

Western Blot: CCT5 Antibody [NBP1-31337] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry: CCT5 Antibody [NBP1-31337] - Immunohistochemical analysis of frozen sections of zebrafish muscle, 5 hpf, using CCT5 antibody [N1N3] (NBP1-31337) at 1:100 dilution.

• Reactivity: Hu, Mu, Ze, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CCT5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CCT5 Antibody - BSA Free (NBP1-31337) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CCT5. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCT5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Monkey (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CCT5 Antibody - BSA Free

• chaperonin containing TCP1, subunit 5 (epsilon)
• epsilon subunit

Background

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CCT5