CCNDBP1 Antibody

Immunohistochemistry-Paraffin: CCNDBP1 Antibody [NB100-1133] - staining.

• Reactivity: Hu, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CCNDBP1 Antibody Summary

• Description: Novus Biologicals Goat CCNDBP1 Antibody (NB100-1133) is a polyclonal antibody validated for use in IHC and ELISA. Anti-CCNDBP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NRIKELTQSELEL corresponding to C-Terminus according to NP_036274.3, NP_411241.1.
• Specificity: This antibody is expected to recognise both human isoforms of this protein.
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCNDBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 55 kDa band in K562 lysate at 0.5 ug/ml, this band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in scientific literature for the band we observe given the predicted MW of approx. 41 kDa and 27 kDa band according to NP_036274; NP_411241. IHC-P: Human prostate shows textured cytoplasm staining with emphasis on the luminal side of the secretory cells in the glands.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CCNDBP1 Antibody

• cyclin D-type binding-protein 1
• cyclin-D1-binding protein 1
• DIP1HHM Protein
• D-type cyclin-interacting protein 1
• GCIPgrap2 cyclin interacting protein
• Grap2 and cyclin-D-interacting protein
• HHM
• Human homolog of Maid
• MAID protein

Background

CCNDBP1 was identified by the interaction of its gene product with Grap2, a leukocyte-specific adaptor protein important for immune cell signaling. The protein encoded by this gene was shown to interact with cyclin D. Transfection of this gene in cells was reported to reduce the phosphorylation of Rb gene product by cyclin D-dependent protein kinase, and inhibit E2F1-mediated transcription activity. This protein was also found to interact with helix-loop-helix protein E12 and is thought to be a negative regulator of liver-specific gene expression. Two alternatively spliced variants, which encode distinct isoforms, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCNDBP1 Antibody (NB100-1133)(1)

Publication using NB100-1133

• Xia C, Bao Z, Tabassam F et al. GCIP, a novel human grap2 and cyclin D interacting protein, regulates E2F-mediated transcriptional activity. J Biol Chem 2000-07-07 [PMID: 10801854]

Bioinformatics

• Gene Symbol: CCNDBP1
• Entrez:
  • 23582(Human)
• Uniprot:
  • NP_036274(Human)