CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free

Western Blot: CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free [NBP2-72106] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNB1IP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNB1IP1.

Immunohistochemistry: CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free [NBP2-72106] - Staining of paraffin-embedded Human pancreas tissue using anti-CCNB1IP1 mouse monoclonal antibody.

Flow Cytometry: CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free [NBP2-72106] - Analysis of Jurkat cells, using anti-CCNB1IP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry: CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free [NBP2-72106] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCNB1IP1 mouse monoclonal antibody.

Immunohistochemistry: CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free [NBP2-72106] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CCNB1IP1 mouse monoclonal antibody.

Immunohistochemistry: CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free [NBP2-72106] - Staining of paraffin-embedded Human lymph node tissue using anti-CCNB1IP1 mouse monoclonal antibody.

Immunohistochemistry: CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free [NBP2-72106] - Staining of paraffin-embedded Human prostate tissue using anti-CCNB1IP1 mouse monoclonal antibody.

Flow Cytometry: CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free [NBP2-72106] - Analysis of Hela cells, using anti-CCNB1IP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI4H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free (NBP2-00600) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CCNB1IP1 (NP_878269) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCNB1IP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 31.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for CCNB1IP1 Antibody (OTI4H3) - Azide and BSA Free

• chromosome 14 open reading frame 18
• cyclin B1 interacting protein 1
• cyclin B1 interacting protein 1, E3 ubiquitin protein ligase
• E3 ubiquitin-protein ligase CCNB1IP1
• EC 6.3.2.-
• enhancer of invasion 10
• HEI10C14orf18Cyclin-B1-interacting protein 1
• Human enhancer of invasion 10

Background

HEI10 is a member of the E3 ubiquitin ligase family and functions in progression of the cell cycle through G(2)/M.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCNB1IP1