CCL8/MCP-2 Antibody (CCL8/3683) [PE]

Flow Cytometry: Mouse CCL8/MCP-2 mAb (CCL8/3683) [PE] [NBP3-14173PE] - NK cells were isolated and stimulated with target cells in an effector: target ratio of 2:1. Left graph depicts unstimulated cells, stimulated cells on the right. Image from a verified customer review.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: CCL8/3683
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

CCL8/MCP-2 Antibody (CCL8/3683) [PE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Human recombinant CCL8/MCP-2 protein fragment (around aa1-99) (exact sequence is proprietary) (Uniprot: P80075)
• Localization: Secreted
• Isotype: IgG2c Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL8
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Validated for Flow Cytometry from a verified customer review.
  • Immunohistochemistry-Paraffin
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP3-14173. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for CCL8/MCP-2 Antibody (CCL8/3683) [PE]

• C-C motif chemokine 8
• CCL8
• chemokine (C-C motif) ligand 8
• HC14
• MCP2
• MCP-2
• member 8 (monocyte chemotactic protein 2)
• monocyte chemoattractant protein 2
• SCYA10
• small-inducible cytokine A8

Background

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CCL8/MCP-2 Antibody (NBP3-14173PE) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Luis Romero Martin Flow Human 04/03/2025

Summary

• Application: Flow Cytometry
• Sample Tested: Natural killer cells
• Species: Human
• Lot: D185052 18-09-2025

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: CCL8