CCL8/MCP-2 Antibody (35509) [Unconjugated]

281-CP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/MCP-2 (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL8/MCP-2 Monoclonal Antibody (Catalog # MAB281). The ND₅₀ is typically 1-4 µg/mL." > 281-CP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/MCP-2 (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL8/MCP-2 Monoclonal Antibody (Catalog # MAB281). The ND₅₀ is typically 1-4 µg/mL." title="Recombinant Human CCL8/MCP-2 (Catalog # 281-CP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/MCP-2 (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL8/MCP-2 Monoclonal Antibody (Catalog # MAB281). The ND₅₀ is typically 1-4 µg/mL." />

Recombinant Human CCL8/MCP-2 (Catalog # <a class=

281-CP) was serially diluted and captured by Mouse Anti-Human CCL8/MCP‑2 Monoclonal Antibody (Catalog # MAB281) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL8/MCP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-281-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 281-CP) was serially diluted and captured by Mouse Anti-Human CCL8/MCP‑2 Monoclonal Antibody (Catalog # MAB281) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL8/MCP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-281-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP) was serially diluted and captured by Mouse Anti-Human CCL8/MCP‑2 Monoclonal Antibody (Catalog # MAB281) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL8/MCP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-281-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human CCL8/MCP‑2 (Catalog # <a class=

• Reactivity: Hu
• Applications: B/N, ELISA(Cap)
• Clone: 35509
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL8/MCP-2 Antibody (35509) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL8/MCP-2
• Specificity: Detects human CCL8/MCP-2 in ELISAs. In ELISAs, this antibody shows less than 0.1% cross-reactivity with recombinant human (rh) CCL15 and rmCCL3.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL8
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 1-4 ug/mL
• Application Notes: ELISA Detection: Human CCL8/MCP-2 Biotinylated Antibody (Catalog number BAF281)
  Standard: Recombinant Human CCL8/MCP-2 (Catalog number 281-CP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL8/MCP-2 Antibody (35509) [Unconjugated]

• C-C motif chemokine 8
• CCL8
• chemokine (C-C motif) ligand 8
• HC14
• MCP2
• MCP-2
• member 8 (monocyte chemotactic protein 2)
• monocyte chemoattractant protein 2
• SCYA10
• small-inducible cytokine A8

Background

CCL8, also known as Monocyte Chemoattractant Protein 2 (MCP-2), is an inflammatory CC chemokine that attracts monocytes, eosinophils and basophils. It is produced by many cell types and signals through interactions with CCR1, CCR2, CCR3 and CCR5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL8/MCP-2 Antibody (MAB281)(7)

Publications using MAB281

• Parween, F;Singh, SP;Kathuria, N;Zhang, HH;Ashida, S;Otaizo-Carrasquero, FA;Shamsaddini, A;Gardina, PJ;Ganesan, S;Kabat, J;Lorenzi, HA;Riley, DJ;Myers, TG;Pittaluga, S;Bielekova, B;Farber, JM; Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokines Nature communications 2025-02-26 [PMID: 40000641] (Immunocytochemistry, Human)
• F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-02-13;0(0):. 2023-02-13 [PMID: 36789428] (ICC, Human)
• Manning L, Cutts J, Stanisic D, Laman M, Carmagnac A, Allen S, O'Donnell A, Karunajeewa H, Rosanas-Urgell A, Siba P, Davis T, Michon P, Schofield L, Rockett K, Kwiatkowski D, Mueller I A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children. Genes Immun, 2015-12-03;17(1):52-9. 2015-12-03 [PMID: 26633000] (ELISA Development (Capture), Human)
• Karta , Maya R, Wickert , Lisa E, Curran , Colleen, Gavala , Monica L, Denlinger , Loren C, Gern , James E, Bertics , Paul J Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages. J Allergy Clin Immunol, 2014-02-14;133(4):1227-30. 2014-02-14 [PMID: 24529411] (ELISA Development (Capture), Human)
• Struyf S, Proost P, Vandercappellen J Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects. Eur. J. Immunol., 2009-03-01;39(3):843-57. 2009-03-01 [PMID: 19224633] (ELISA Development, Human)
• Haringman JJ, Smeets TJ, Reinders-Blankert P Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis. Ann. Rheum. Dis., 2005-08-17;65(3):294-300. 2005-08-17 [PMID: 16107514] (IHC-Fr, Human)
• Tay SS, McCormack A, Rose ML Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production. Transplantation, 2004-10-15;78(7):987-94. 2004-10-15 [PMID: 15480163] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CCL8