CCL8/MCP-2 Antibody (35509.11) [FITC]

Immunocytochemistry/Immunofluorescence: CCL8/MCP-2 Antibody (35509.11) [FITC] [NB120-10391F] - Panel shows intracellular staining to measure CCL8/MCP-2 (NB120-10391F) levels in untreated or treated MDDCs. Panel shows representative single cell images. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28894190/) licensed under a CC-BY license.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: 35509.11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

CCL8/MCP-2 Antibody (35509.11) [FITC] Summary

• Immunogen: Recombinant human monocyte chemotactic protein 2 (MCP2), expressed in E. coli.
• Localization: Secreted
• Specificity: By ELISA, this antibody shows cross-reactivity with recombinant human MCP1 and recombinat mouse MIP1 alpha. In addition, it does not cross-react with other chemokines tested: rmC10, rhENA78, rhGRO alpha, rhGRO beta, rhGRO gamma, rmJE, rhI309, and rhIL8.
  
  This antibody neutralizes the biological activity of recombinant human MCP-2. Shows <20% cross-reactivity with recombinant human MCP-1 and recombinant mouse MIP-1a.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CCL8/MCP-2 Antibody (35509.11) [FITC]

• C-C motif chemokine 8
• CCL8
• chemokine (C-C motif) ligand 8
• HC14
• MCP2
• MCP-2
• member 8 (monocyte chemotactic protein 2)
• monocyte chemoattractant protein 2
• SCYA10
• small-inducible cytokine A8

Background

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL8/MCP-2 Antibody (NB120-10391F)(1)

Publication using NB120-10391F

• Parira T, Figueroa G, et al. Novel detection of post-translational modifications in human monocyte-derived dendritic cells after chronic alcohol exposure: Role of inflammation regulator H4K12ac. Sci Rep 2017-09-11 [PMID: 28894190] (FLOW, Human)

Bioinformatics

• Gene Symbol: CCL8
• Entrez:
  • 6355(Human)
  • 20307(Mouse)