CCL8/MCP-2 Antibody (35509.11)

Immunocytochemistry/Immunofluorescence: CCL8/MCP-2 Antibody (35509.11) [NB120-10391] - Panel shows intracellular staining to measure CCL8/MCP-2 levels in untreated or treated MDDCs. Panel shows representative single cell images. Analysis using FITC conjugated version of antibody (NB120-10391F). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28894190/) licensed under a CC-BY license.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, B/N
• Clone: 35509.11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL8/MCP-2 Antibody (35509.11) Summary

• Immunogen: Recombinant human monocyte chemotactic protein 2 (MCP2), expressed in E. coli.
• Localization: Secreted
• Specificity: By ELISA, this antibody shows cross-reactivity with recombinant human MCP1 and recombinat mouse MIP1 alpha. In addition, it does not cross-react with other chemokines tested: rmC10, rhENA78, rhGRO alpha, rhGRO beta, rhGRO gamma, rmJE, rhI309, and rhIL8.
  
  This antibody neutralizes the biological activity of recombinant human MCP-2. Shows <20% cross-reactivity with recombinant human MCP-1 and recombinant mouse MIP-1a.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1 - 2 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Neutralization 1 - 4 ug/ml
  • Western Blot 1 - 2 ug/ml
• Application Notes: ELISA: Use at a concentration of 0.5 - 1 ug/ml to detect 12.5ng/ml of recombinant human MCP2.Neut: Use at a concentration of 0.5 - 10 ug/ml. WB: Use at a concentration of 1 - 2 ug/ml to detect 50ng/lane of recombinant human MCP2 under reducing and non-reducing conditions. Predicted molecular weight: 9 kDa. Human MCP2 is tested for its ability to neutralize the chemotactic activity of rhMCP2 for human monocytes. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhMCP2 that is present at a concentration just high enough to elicit a maximum response. In this bioassay 0.4ug/ml rhMCP2 was mixed with various dilutions of the antibody for 30 minutes at 22.
  
  Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 28894190).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered PBS and 5% Trehalose
• Preservative: 5% Trehalose
• Purity: Protein A purified

Alternate Names for CCL8/MCP-2 Antibody (35509.11)

• C-C motif chemokine 8
• CCL8
• chemokine (C-C motif) ligand 8
• HC14
• MCP2
• MCP-2
• member 8 (monocyte chemotactic protein 2)
• monocyte chemoattractant protein 2
• SCYA10
• small-inducible cytokine A8

Background

MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL8/MCP-2 Antibody (NB120-10391)(2)

Publications using NB120-10391

• Lee J, Lee y, Kim KH et al. Chemokine (C-C Motif) Ligand 8 and Tubulo-Interstitial Injury in Chronic Kidney Disease Cells 2022-02-14 [PMID: 35203308] (WB, IHC-P, Human)
• Parira T, Figueroa G, et al. Novel detection of post-translational modifications in human monocyte-derived dendritic cells after chronic alcohol exposure: Role of inflammation regulator H4K12ac. Sci Rep 2017-09-11 [PMID: 28894190] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CCL8
• Entrez:
  • 6355(Human)
  • 20307(Mouse)