CCL28 Antibody (62714) [Unconjugated]

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 62714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CCL28 Antibody (62714) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CCL28
  Ile23-Tyr127
  Accession # Q9NRJ3
• Specificity: Detects human CCL28 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL27 or recombinant mouse CCL28 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL28
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human CCL28 Biotinylated Antibody (Catalog number BAF717)
  Standard: Recombinant Human CCL28 (Catalog number 717-VC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL28 Antibody (62714) [Unconjugated]

• CCL28
• chemokine (C-C motif) ligand 28
• MEC
• member 28
• MGC71902
• Protein CCK1
• VIC

Background

Human CCL28 (CC chemokine ligand 28) is a member of the CC chemokine superfamily. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 share 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. Human and mouse CCL28 RNA is most highly expressed by epithelial cells in normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. The receptor for CCL28 is the CCR10 (formerly GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL28 Antibody (MAB7171)(3)

Publications using MAB7171

• M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals Mucosal Immunol, 2020-04-28;0(0):. 2020-04-28 [PMID: 32346082] (IHC, Human)
• Ishan Roy, Kathleen A. Boyle, Emily P. Vonderhaar, Noah P. Zimmerman, Egal Gorse, A. Craig Mackinnon et al. Cancer cell chemokines direct chemotaxis of activated stellate cells in pancreatic ductal adenocarcinoma Laboratory Investigation 2017-03-01 [PMID: 28092365] (Immunohistochemistry, Human)
• Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy. Clin Cancer Res, 2015-02-23;21(12):2840-50. 2015-02-23 [PMID: 25712684] (IHC, Neutralization, Human)

Bioinformatics

• Gene Symbol: CCL28
• Uniprot:
  • Q9NRJ3()