CCL28 Antibody (62705) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clone
62705
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

CCL28 Antibody (62705) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL28
Ile23-Tyr127
Accession # Q9NRJ3
Specificity
Detects human CCL28 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 19, 21, 22, 25, or recombinant rat CCL20 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL28
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Neutralization 15-45 ug/mL
Publications
Read Publications using
MAB717 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL28 Antibody (62705) [Unconjugated]

  • CCL28
  • chemokine (C-C motif) ligand 28
  • MEC
  • member 28
  • MGC71902
  • Protein CCK1
  • VIC

Background

Human CCL28 (CC chemokine ligand 28) is a 127 amino acid (aa) precursor protein with a putative 22 aa signal peptide that is cleaved to produce the 105 aa residue mature protein. Mature human and mouse CCL28  share 83% aa sequence identity. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression is highest in epithelial cells of normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. CCR10 (GPR2 orphan receptor) is the receptor for both CCL27/CTACK and  CCL28.

  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL28 Antibody (MAB717)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA Development, Functional Assay, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
Functional Assay
(1)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using MAB717 Applications Species
M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals Mucosal Immunol, 2020;0(0):. 2020 [PMID: 32346082] (Functional Assay, Human) Functional Assay Human
E Esposito, BJ Ahn, J Shi, Y Nakamura, JH Park, ET Mandeville, Z Yu, SJ Chan, R Desai, A Hayakawa, X Ji, EH Lo, K Hayakawa Brain-to-cervical lymph node signaling after stroke Nat Commun, 2019;10(1):5306. 2019 [PMID: 31757960] (Western Blot, Mouse) Western Blot Mouse
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011;475(7355):226-30. 2011 [PMID: 21753853] (Neutralization, Human) Neutralization Human
Eksteen B, Miles A, Curbishley SM, Tselepis C, Grant AJ, Walker LS, Adams DH Epithelial inflammation is associated with CCL28 production and the recruitment of regulatory T cells expressing CCR10. J. Immunol., 2006;177(1):593-603. 2006 [PMID: 16785557] (Western Blot, Human) Western Blot Human
Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. J. Immunol., 2003;170(3):1452-61. 2003 [PMID: 12538707] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol CCL28
Uniprot